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Z-scores for comparative analyses of spermatogonial numbers throughout human development.
- Source :
-
Fertility and sterility [Fertil Steril] 2021 Sep; Vol. 116 (3), pp. 713-720. Date of Electronic Publication: 2021 May 08. - Publication Year :
- 2021
-
Abstract
- Objective: To normalize age-dependent effects on standardized measures of spermatogonial quantity such as the number of spermatogonia per tubular cross-section (S/T) or fertility index.<br />Design: Published quantitative histologic data on human spermatogonial numbers were used to create Z-scores for reference means and tested on archived testicular tissue samples.<br />Setting: Retrospective cohort study.<br />Patient(s): The sample cohort comprised testicular samples from 24 boys with cancer diagnosis and 10 with Klinefelter syndrome, as part of the fertility preservation programs NORDFERTIL and Androprotect, as well as archived histologic samples from 35 prepubertal boys with acute lymphoblastic leukemia and 20 testicular biobank samples.<br />Intervention(s): None.<br />Main Outcome Measure(s): Z-score values for S/T and fertility index on the basis of morphology and germ cell-specific markers (MAGEA4 and/or DDX4) were calculated, and the impact of cancer therapy exposure and genetic disorders on Z-score values was evaluated.<br />Result(s): The Z-scores for S/T values in the nontreated samples (-2.08 ± 2.20, n = 28) and samples treated with nonalkylating agents (-1.90 ± 2.60, n = 25) were comparable within ±3 standard deviations of the reference mean value but differed significantly from samples exposed to alkylating agents (-12.14 ± 9.20, n = 22) and from patients with Klinefelter syndrome (-11.56 ± 4.89, n = 8). The Z-scores for S/T were correlated with increasing cumulative exposure to alkylating agents (r = -0.7020).<br />Conclusion(s): The Z-score values for S/T allow for the quantification of genetic and cancer treatment-related effects on testicular tissue stored for fertility preservation, facilitating their use for patient counseling.<br /> (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Adolescent
Age Factors
Child
Child, Preschool
Fertility drug effects
Fertility Preservation
Humans
Infertility, Male pathology
Infertility, Male physiopathology
Infertility, Male therapy
Klinefelter Syndrome complications
Male
Retrospective Studies
Risk Factors
Sperm Count
Spermatogonia pathology
Adolescent Development
Antineoplastic Agents adverse effects
Child Development
Infertility, Male chemically induced
Klinefelter Syndrome pathology
Precursor Cell Lymphoblastic Leukemia-Lymphoma drug therapy
Spermatogonia drug effects
Testicular Neoplasms drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1556-5653
- Volume :
- 116
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Fertility and sterility
- Publication Type :
- Academic Journal
- Accession number :
- 33975728
- Full Text :
- https://doi.org/10.1016/j.fertnstert.2021.04.019