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A common F-box gene regulates the leucine homeostasis of Medicago truncatula and Arabidopsis thaliana.

Authors :
Iantcheva A
Zhiponova M
Revalska M
Heyman J
Dincheva I
Badjakov I
De Geyter N
Boycheva I
Goormachtig S
De Veylder L
Source :
Protoplasma [Protoplasma] 2022 Mar; Vol. 259 (2), pp. 277-290. Date of Electronic Publication: 2021 May 10.
Publication Year :
2022

Abstract

The F-box domain is a conserved structural protein motif that most frequently interacts with the SKP1 protein, the core of the SCFs (SKP1-CULLIN-F-box protein ligase) E3 ubiquitin protein ligases. As part of the SCF complexes, the various F-box proteins recruit substrates for degradation through ubiquitination. In this study, we functionally characterized an F-box gene (MtF-box) identified earlier in a population of Tnt1 retrotransposon-tagged mutants of Medicago truncatula and its Arabidopsis thaliana homolog (AtF-box) using gain- and loss-of-function plants. We highlighted the importance of MtF-box in leaf development of M. truncatula. Protein-protein interaction analyses revealed the 2-isopropylmalate synthase (IPMS) protein as a common interactor partner of MtF-box and AtF-box, being a key enzyme in the biosynthesis pathway of the branched-chain amino acid leucine. For further detailed analysis, we focused on AtF-box and its role during the cell division cycle. Based on this work, we suggest a mechanism for the role of the studied F-box gene in regulation of leucine homeostasis, which is important for growth.<br /> (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)

Details

Language :
English
ISSN :
1615-6102
Volume :
259
Issue :
2
Database :
MEDLINE
Journal :
Protoplasma
Publication Type :
Academic Journal
Accession number :
33973099
Full Text :
https://doi.org/10.1007/s00709-021-01662-w