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Capturing T Lymphocytes' Dynamic Interactions With Human Neural Cells Using Time-Lapse Microscopy.
- Source :
-
Frontiers in immunology [Front Immunol] 2021 Apr 22; Vol. 12, pp. 668483. Date of Electronic Publication: 2021 Apr 22 (Print Publication: 2021). - Publication Year :
- 2021
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Abstract
- To fully perform their functions, T lymphocytes migrate within organs' parenchyma and interact with local cells. Infiltration of T lymphocytes within the central nervous system (CNS) is associated with numerous neurodegenerative disorders. Nevertheless, how these immune cells communicate and respond to neural cells remains unresolved. To investigate the behavior of T lymphocytes that reach the CNS, we have established an in vitro co-culture model and analyzed the spatiotemporal interactions between human activated CD8 <superscript>+</superscript> T lymphocytes and primary human astrocytes and neurons using time-lapse microscopy. By combining multiple variables extracted from individual CD8 <superscript>+</superscript> T cell tracking, we show that CD8 <superscript>+</superscript> T lymphocytes adopt a more motile and exploratory behavior upon interacting with astrocytes than with neurons. Pretreatment of astrocytes or neurons with IL-1β to mimic in vivo inflammation significantly increases CD8 <superscript>+</superscript> T lymphocyte motility. Using visual interpretation and analysis of numerical variables extracted from CD8 <superscript>+</superscript> T cell tracking, we identified four distinct CD8 <superscript>+</superscript> T lymphocyte behaviors: scanning, dancing, poking and round. IL-1β-pretreatment significantly increases the proportion of scanning CD8 <superscript>+</superscript> T lymphocytes, which are characterized by active exploration, and reduces the proportion of round CD8 <superscript>+</superscript> T lymphocytes, which are less active. Blocking MHC class I on astrocytes significantly diminishes the proportion of poking CD8 <superscript>+</superscript> T lymphocytes, which exhibit synapse-like interactions. Lastly, our co-culture time-lapse model is easily adaptable and sufficiently sensitive and powerful to characterize and quantify spatiotemporal interactions between human T lymphocytes and primary human cells in different conditions while preserving viability of fragile cells such as neurons and astrocytes.<br />Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.<br /> (Copyright © 2021 Lemaître, Carmena Moratalla, Farzam-kia, Carpentier Solorio, Tastet, Cleret-Buhot, Guimond, Haddad and Arbour.)
- Subjects :
- Adult
Astrocytes drug effects
Astrocytes immunology
CD8-Positive T-Lymphocytes immunology
Cell Movement
Cells, Cultured
Coculture Techniques
Female
Histocompatibility Antigens Class I immunology
Humans
Inflammation immunology
Interleukin-1beta pharmacology
Male
Middle Aged
Neurons drug effects
Neurons immunology
Phenotype
Time Factors
Young Adult
Astrocytes physiology
CD8-Positive T-Lymphocytes physiology
Cell Communication drug effects
Microscopy, Video
Neurons metabolism
Time-Lapse Imaging
Subjects
Details
- Language :
- English
- ISSN :
- 1664-3224
- Volume :
- 12
- Database :
- MEDLINE
- Journal :
- Frontiers in immunology
- Publication Type :
- Academic Journal
- Accession number :
- 33968073
- Full Text :
- https://doi.org/10.3389/fimmu.2021.668483