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5 ns electric pulses induce Ca 2+ -dependent exocytotic release of catecholamine from adrenal chromaffin cells.

Authors :
Zaklit J
Cabrera A
Shaw A
Aoun R
Vernier PT
Leblanc N
Craviso GL
Source :
Bioelectrochemistry (Amsterdam, Netherlands) [Bioelectrochemistry] 2021 Aug; Vol. 140, pp. 107830. Date of Electronic Publication: 2021 Apr 27.
Publication Year :
2021

Abstract

Previously we reported that adrenal chromaffin cells exposed to a 5 ns, 5 MV/m pulse release the catecholamines norepinephrine (NE) and epinephrine (EPI) in a Ca <superscript>2+</superscript> -dependent manner. Here we determined that NE and EPI release increased with pulse number (one versus five and ten pulses at 1 Hz), established that release occurs by exocytosis, and characterized the exocytotic response in real-time. Evidence of an exocytotic mechanism was the appearance of dopamine-β-hydroxylase on the plasma membrane, and the demonstration by total internal reflection fluorescence microscopy studies that a train of five or ten pulses at 1 Hz triggered the release of the fluorescent dye acridine orange from secretory granules. Release events were Ca <superscript>2+</superscript> -dependent, longer-lived relative to those evoked by nicotinic receptor stimulation, and occurred with a delay of several seconds despite an immediate rise in Ca <superscript>2+</superscript> . In complementary studies, cells labeled with the plasma membrane fluorescent dye FM 1-43 and exposed to a train of ten pulses at 1 Hz underwent Ca <superscript>2+</superscript> -dependent increases in FM 1-43 fluorescence indicative of granule fusion with the plasma membrane due to exocytosis. These results demonstrate the effectiveness of ultrashort electric pulses for stimulating catecholamine release, signifying their promise as a novel electrostimulation modality for neurosecretion.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2021 Elsevier B.V. All rights reserved.)

Details

Language :
English
ISSN :
1878-562X
Volume :
140
Database :
MEDLINE
Journal :
Bioelectrochemistry (Amsterdam, Netherlands)
Publication Type :
Academic Journal
Accession number :
33965669
Full Text :
https://doi.org/10.1016/j.bioelechem.2021.107830