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Synthase-Selective Exploration of a Tunicate Microbiome by Activity-Guided Single-Cell Genomics.
- Source :
-
ACS chemical biology [ACS Chem Biol] 2021 May 21; Vol. 16 (5), pp. 813-819. Date of Electronic Publication: 2021 May 06. - Publication Year :
- 2021
-
Abstract
- While thousands of environmental metagenomes have been mined for the presence of novel biosynthetic gene clusters, such computational predictions do not provide evidence of their in vivo biosynthetic functionality. Using fluorescent in situ enzyme assay targeting carrier proteins common to polyketide (PKS) and nonribosomal peptide synthetases (NRPS), we applied fluorescence-activated cell sorting to tunicate microbiome to enrich for microbes with active secondary metabolic capabilities. Single-cell genomics uncovered the genetic basis for a wide biosynthetic diversity in the enzyme-active cells and revealed a member of marine Oceanospirillales harboring a novel NRPS gene cluster with high similarity to phylogenetically distant marine and terrestrial bacteria. Interestingly, this synthase belongs to a larger class of siderophore biosynthetic gene clusters commonly associated with pestilence and disease. This demonstrates activity-guided single-cell genomics as a tool to guide novel biosynthetic discovery.
- Subjects :
- Animals
Bacillus subtilis genetics
Carrier Proteins chemistry
Carrier Proteins genetics
Ciona intestinalis metabolism
Computational Biology
Escherichia coli genetics
Flow Cytometry
Phylogeny
Polyketides chemistry
Secondary Metabolism
Siderophores chemistry
Single-Cell Analysis
Genomics methods
Microbiota genetics
Multigene Family genetics
Peptide Synthases metabolism
Siderophores genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1554-8937
- Volume :
- 16
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- ACS chemical biology
- Publication Type :
- Academic Journal
- Accession number :
- 33955744
- Full Text :
- https://doi.org/10.1021/acschembio.1c00157