Nanobiocatalysts for efficacious bioconversion of ionic liquid pretreated sugarcane tops biomass to biofuel.

Authors :: Sharma S
Nargotra P
Sharma V
Bangotra R
Kaur M
Kapoor N
Paul S
Bajaj BK
Source :: Bioresource technology [Bioresour Technol] 2021 Aug; Vol. 333, pp. 125191. Date of Electronic Publication: 2021 Apr 20.
Publication Year :: 2021
Abstract: This work aimed to study the hydrolysis of ionic liquid (IL) pretreated sugarcane tops (SCT) biomass with in-house developed IL-stable enzyme preparation, from a fungal isolate Aspergillus flavus PN3. Maximum reducing sugar yield (181.18 mg/g biomass) was obtained from tris (2-hydroxyethyl) methylammonium-methylsulfate ([TMA]MeSO <subscript>4</subscript> ) pretreated biomass. Pretreatment parameters were optimized to attain enhanced sugar yield (1.57-fold). Functional mechanism of IL mediated pretreatment of SCT biomass was elucidated by SEM, XRD, FTIR and <superscript>1</superscript> H NMR studies. Furthermore, nanobiocatalysts prepared by immobilization of enzyme preparation by covalent coupling on magnetic nanoparticles functionalized with amino-propyl triethoxysilane, were assessed for their hydrolytic efficacy and reusability. Nanobiocatalysts were examined by SEM and FTIR analysis for substantiation of immobilization. This is the first ever report of application of magnetic nanobiocatalysts for saccharification of IL-pretreated sugarcane tops biomass.<br /> (Copyright © 2021 Elsevier Ltd. All rights reserved.)

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