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Immune Polarization Potential of the S. aureus Virulence Factors SplB and GlpQ and Modulation by Adjuvants.
- Source :
-
Frontiers in immunology [Front Immunol] 2021 Apr 15; Vol. 12, pp. 642802. Date of Electronic Publication: 2021 Apr 15 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- Protection against Staphylococcus aureus is determined by the polarization of the anti-bacterial immune effector mechanisms. Virulence factors of S. aureus can modulate these and induce differently polarized immune responses in a single individual. We proposed that this may be due to intrinsic properties of the bacterial proteins. To test this idea, we selected two virulence factors, the serine protease-like protein B (SplB) and the glycerophosphoryl diester phosphodiesterase (GlpQ). In humans naturally exposed to S. aureus , SplB induces a type 2-biased adaptive immune response, whereas GlpQ elicits type 1/type 3 immunity. We injected the recombinant bacterial antigens into the peritoneum of S. aureus -naïve C57BL/6N mice and analyzed the immune response. This was skewed by SplB toward a Th2 profile including specific IgE, whereas GlpQ was weakly immunogenic. To elucidate the influence of adjuvants on the proteins' polarization potential, we studied Montanide ISA 71 VG and Imject™Alum, which promote a Th1 and Th2 response, respectively. Alum strongly increased antibody production to the Th2-polarizing protein SplB, but did not affect the response to GlpQ. Montanide enhanced the antibody production to both S. aureus virulence factors. Montanide also augmented the inflammation in general, whereas Alum had little effect on the cellular immune response. The adjuvants did not override the polarization potential of the S. aureus proteins on the adaptive immune response.<br />Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.<br /> (Copyright © 2021 Mrochen, Trübe, Jorde, Domanska, Brandt and Bröker.)
- Subjects :
- Animals
Antibodies, Bacterial blood
Antigen-Presenting Cells immunology
Cytokines biosynthesis
Female
Immunization
Mice
Mice, Inbred C57BL
Th1 Cells immunology
Th2 Cells immunology
Adjuvants, Immunologic pharmacology
Antigens, Bacterial immunology
Bacterial Proteins immunology
Phosphoric Diester Hydrolases immunology
Staphylococcus aureus immunology
Virulence Factors immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1664-3224
- Volume :
- 12
- Database :
- MEDLINE
- Journal :
- Frontiers in immunology
- Publication Type :
- Academic Journal
- Accession number :
- 33936060
- Full Text :
- https://doi.org/10.3389/fimmu.2021.642802