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Diagnostic accuracy of the FluoroType MTB and MTBDR VER 2.0 assays for the centralized high-throughput detection of Mycobacterium tuberculosis complex DNA and isoniazid and rifampicin resistance.
- Source :
-
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases [Clin Microbiol Infect] 2021 Sep; Vol. 27 (9), pp. 1351.e1-1351.e4. Date of Electronic Publication: 2021 Apr 30. - Publication Year :
- 2021
-
Abstract
- Objectives: To evaluate the accuracy of two new molecular diagnostic tests for the detection of drug-resistant tuberculosis, the FluoroType MTB and MTBDR VER 2.0 assays, in combination with manual and automated DNA extraction methods.<br />Methods: Sputa from 360 Xpert Ultra Mycobacterium tuberculosis complex (MTBC)-positive patients and 250 Xpert Ultra MTBC-negative patients were tested. GenoType MTBDRplus served as reference for MTBC and drug resistance detection. Sanger sequencing was used to resolve discrepancies.<br />Results: FluoroType MTB VER 2.0 showed similar MTBC sensitivity compared with FluoroType MTBDR VER 2.0 (manual DNA extraction: 91.6% (294/321) versus 89.8% (291/324); p 0.4); automated DNA extraction: 92.1% (305/331) versus 87.7% (291/332); p 0.05)). FluoroType MTBDR VER2.0 showed comparable diagnostic accuracy to FluoroType MTBDR VER1.0 as previously reported for the detection of MTBC and rifampicin and isoniazid resistance.<br />Conclusions: The FluoroType MTB and MTBDR VER 2.0 assays together with an automated DNA extraction and PCR set-up platform may improve laboratory operational efficiency for the diagnosis of MTBC and resistance to rifampicin and isoniazid and show promise for the implementation in a centralized molecular drug susceptibility testing model.<br /> (Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
- Subjects :
- Antitubercular Agents pharmacology
DNA, Bacterial isolation & purification
Humans
Molecular Diagnostic Techniques
Sensitivity and Specificity
Tuberculosis diagnosis
Tuberculosis microbiology
Drug Resistance, Bacterial
Isoniazid pharmacology
Microbial Sensitivity Tests
Mycobacterium tuberculosis drug effects
Mycobacterium tuberculosis genetics
Rifampin pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1469-0691
- Volume :
- 27
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 33933566
- Full Text :
- https://doi.org/10.1016/j.cmi.2021.04.022