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Stick-Based Methods for Aptamer-Mediated siRNA Targeted Delivery.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2282, pp. 31-42. - Publication Year :
- 2021
-
Abstract
- Despite the therapeutic utility of small interfering RNA (siRNA) molecules, the development of a safe and reliable method to selectively target diseased organs and tissues is still a critical need for their translation to the clinic. Here we describe how nucleic acid-based aptamers against cell surface epitopes may be used to address this issue. We discuss the most recent examples and advances in the field of aptamer siRNA delivery and provide a fast and simple protocol for the design and generation of aptamer-siRNA chimeras. The described approach is based on the annealing of the targeting aptamer, and the antisense strand through "stick" complementary sequences elongated at their 3' end, and the subsequent paring with the sense strand. Such a protocol allows a modular non-covalent generation of the constructs and permits an efficient delivery of the siRNA moiety into aptamer target cells.
- Subjects :
- Aptamers, Nucleotide metabolism
Brain Neoplasms genetics
Brain Neoplasms metabolism
Cell Line, Tumor
Gene Expression Regulation, Neoplastic
Glioblastoma genetics
Glioblastoma metabolism
Humans
RNA, Small Interfering metabolism
Receptor, Platelet-Derived Growth Factor beta genetics
Receptor, Platelet-Derived Growth Factor beta metabolism
Research Design
STAT3 Transcription Factor metabolism
Workflow
Aptamers, Nucleotide genetics
Gene Transfer Techniques
RNA Interference
RNA, Small Interfering genetics
STAT3 Transcription Factor genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2282
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 33928568
- Full Text :
- https://doi.org/10.1007/978-1-0716-1298-9_3