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Modeling and live imaging of mechanical instabilities in the zebrafish aorta during hematopoiesis.

Authors :
Chalin D
Bureau C
Parmeggiani A
Rochal S
Kissa K
Golushko I
Source :
Scientific reports [Sci Rep] 2021 Apr 29; Vol. 11 (1), pp. 9316. Date of Electronic Publication: 2021 Apr 29.
Publication Year :
2021

Abstract

All blood cells originate from hematopoietic stem/progenitor cells (HSPCs). HSPCs are formed from endothelial cells (ECs) of the dorsal aorta (DA), via endothelial-to-hematopoietic transition (EHT). The zebrafish is a primary model organism to study the process in vivo. While the role of mechanical stress in controlling gene expression promoting cell differentiation is actively investigated, mechanisms driving shape changes of the DA and individual ECs remain poorly understood. We address this problem by developing a new DA micromechanical model and applying it to experimental data on zebrafish morphogenesis. The model considers the DA as an isotropic tubular membrane subjected to hydrostatic blood pressure and axial stress. The DA evolution is described as a movement in the dimensionless controlling parameters space: normalized hydrostatic pressure and axial stress. We argue that HSPC production is accompanied by two mechanical instabilities arising in the system due to the plane stress in the DA walls and show how a complex interplay between mechanical forces in the system drives the emerging morphological changes.

Details

Language :
English
ISSN :
2045-2322
Volume :
11
Issue :
1
Database :
MEDLINE
Journal :
Scientific reports
Publication Type :
Academic Journal
Accession number :
33927284
Full Text :
https://doi.org/10.1038/s41598-021-88667-w