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Noncanonical DNA polymerization by aminoadenine-based siphoviruses.
- Source :
-
Science (New York, N.Y.) [Science] 2021 Apr 30; Vol. 372 (6541), pp. 520-524. - Publication Year :
- 2021
-
Abstract
- Bacteriophage genomes harbor the broadest chemical diversity of nucleobases across all life forms. Certain DNA viruses that infect hosts as diverse as cyanobacteria, proteobacteria, and actinobacteria exhibit wholesale substitution of aminoadenine for adenine, thereby forming three hydrogen bonds with thymine and violating Watson-Crick pairing rules. Aminoadenine-encoded DNA polymerases, homologous to the Klenow fragment of bacterial DNA polymerase I that includes 3'-exonuclease but lacks 5'-exonuclease, were found to preferentially select for aminoadenine instead of adenine in deoxynucleoside triphosphate incorporation templated by thymine. Polymerase genes occur in synteny with genes for a biosynthesis enzyme that produces aminoadenine deoxynucleotides in a wide array of Siphoviridae bacteriophages. Congruent phylogenetic clustering of the polymerases and biosynthesis enzymes suggests that aminoadenine has propagated in DNA alongside adenine since archaic stages of evolution.<br /> (Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.)
- Subjects :
- 2-Aminopurine chemistry
DNA-Directed DNA Polymerase classification
DNA-Directed DNA Polymerase genetics
Genome, Viral
Phylogeny
Siphoviridae genetics
Viral Nonstructural Proteins classification
Viral Nonstructural Proteins genetics
2-Aminopurine analogs & derivatives
DNA Replication
DNA, Viral biosynthesis
DNA-Directed DNA Polymerase chemistry
Polymerization
Siphoviridae chemistry
Siphoviridae enzymology
Viral Nonstructural Proteins chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9203
- Volume :
- 372
- Issue :
- 6541
- Database :
- MEDLINE
- Journal :
- Science (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 33926956
- Full Text :
- https://doi.org/10.1126/science.abe6542