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Platyphyllenone Induces Autophagy and Apoptosis by Modulating the AKT and JNK Mitogen-Activated Protein Kinase Pathways in Oral Cancer Cells.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2021 Apr 19; Vol. 22 (8). Date of Electronic Publication: 2021 Apr 19. - Publication Year :
- 2021
-
Abstract
- Platyphyllenone is a type of diarylheptanoid that exhibits anti-inflammatory and chemoprotective effects. However, its effect on oral cancer remains unclear. In this study, we investigated whether platyphyllenone can promote apoptosis and autophagy in SCC-9 and SCC-47 cells. We found that it dose-dependently promoted the cleavage of PARP; caspase-3, -8, and -9 protein expression; and also led to cell cycle arrest at the G2/M phase. Platyphyllenone up-regulated LC3-II and p62 protein expression in both SCC-9 and SCC-47 cell lines, implying that it can induce autophagy. Furthermore, the results demonstrated that platyphyllenone significantly decreased p-AKT and increased p-JNK1/2 mitogen-activated protein kinase (MAPK) signaling pathway in a dose-dependent manner. The specific inhibitors of p-JNK1/2 also reduced platyphyllenone-induced cleavage of PARP, caspase-3, and caspase -8, LC3-II and p62 protein expression. These findings are the first to demonstrate that platyphyllenone can induce both autophagy and apoptosis in oral cancers, and it is expected to provide a therapeutic option as a chemopreventive agent against oral cancer proliferation.
- Subjects :
- Blotting, Western
Caspase 3
Caspase 8 metabolism
Cell Cycle Checkpoints drug effects
Cell Line, Tumor
Cell Proliferation drug effects
Cell Survival drug effects
Humans
Membrane Potential, Mitochondrial drug effects
Signal Transduction drug effects
Antineoplastic Agents pharmacology
Apoptosis drug effects
Autophagy drug effects
JNK Mitogen-Activated Protein Kinases metabolism
Ketones pharmacology
Mouth Neoplasms metabolism
Proto-Oncogene Proteins c-akt metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 22
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 33921647
- Full Text :
- https://doi.org/10.3390/ijms22084211