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Biogenic copper nanoparticles produced by using the Klebsiella pneumoniae strain NST2 curtailed salt stress effects in maize by modulating the cellular oxidative repair mechanisms.
- Source :
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Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2021 Jul 01; Vol. 217, pp. 112264. Date of Electronic Publication: 2021 Apr 27. - Publication Year :
- 2021
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Abstract
- The negative effects of salinity on plant growth and physiology are well-established, which is one of the major threats to food security in semi-arid and arid regions of the world. The current research focuses on biosynthesis of copper nanoparticles (CuNPs) from a bacterial strain NST2, which was genetically identified as Klebsiella pneumoniae based on taxonomic identity of 16S rRNA gene. The strain was selected for bioprospecting of CuNPs owing to its Cu tolerance potential. The biologically-synthesized CuNPs were confirmed in culture by using ultraviolet visible spectroscopy. The material characteristics of green CuNPs were further investigated by using Fourier transform infrared spectroscopy, X-ray diffractometer, scanning electron microscopy and transmission electron microscopy, where crystallite size was ranged from 22.44 nm to 44.26 nm and particles were stabilized by various functional groups, such as carbonyl and amine groups. When 100 mg kg <superscript>-1</superscript> of green CuNPs were mixed in saline soil in a pot experiment, the maize plants showed increased root and shoot length (43.52% and 44.06%, respectively), fresh weight (46.05% and 51.82%, respectively) and dry weight (47.69% and 30.63%, respectively) in comparison to control maize plants without CuNPs application. Moreover, green CuNPs at their highest treatment level (100 mg kg <superscript>-1</superscript> of soil) counteracted the lipid peroxidation and oxidative damage in maize plants by promoting the activities of antioxidants and demoting the cellular levels of reactive oxygen species and ionic contents of Na <superscript>+</superscript> and Cl <superscript>-</superscript> . Conclusively, biogenic CuNPs is an emerging and promising technique, which could replace traditional methods of salinity management in agricultural soils.<br /> (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Antioxidants pharmacology
Copper chemistry
Klebsiella pneumoniae drug effects
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Oxidative Stress drug effects
RNA, Ribosomal, 16S
Salt Stress drug effects
Spectroscopy, Fourier Transform Infrared
Zea mays drug effects
Copper physiology
Klebsiella pneumoniae physiology
Metal Nanoparticles
Salt Stress physiology
Zea mays physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2414
- Volume :
- 217
- Database :
- MEDLINE
- Journal :
- Ecotoxicology and environmental safety
- Publication Type :
- Academic Journal
- Accession number :
- 33915453
- Full Text :
- https://doi.org/10.1016/j.ecoenv.2021.112264