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Pseudorabies virus pUL16 assists the nuclear import of VP26 through protein-protein interaction.
- Source :
-
Veterinary microbiology [Vet Microbiol] 2021 Jun; Vol. 257, pp. 109080. Date of Electronic Publication: 2021 Apr 20. - Publication Year :
- 2021
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Abstract
- Pseudorabies virus (PRV) is related to alphaherpesvirus and varicellovirus. pUL16 is a conserved protein in all herpesviruses, and studies have shown that UL16 can interact with the viral proteins pUL11, pUL49, pUL21, gD, and gE. In this study, we found that pUL16 interacted with the viral capsid protein VP26, which could not translocate into the nucleus itself but did appear in the nucleus. We further determined whether pUL16 assists the translocation of VP26 into the nucleus. We found that pUL16 interacted with VP26 with or without viral proteins, and since VP26 itself did not contain a nuclear location signal, we concluded that pUL16 assisted the translocation of VP26 into the nucleus. Deletion of UL16 and UL35 significantly reduced the 50 % tissue culture infective dose, virulence, attachment, and internalization of PRV in cells. These results show that the interaction between pUL16 and VP26 influences the growth and virulence of pseudorabies virus. Our research is the first study to show that pUL16 interacts with VP26, which may explain the targeting site of UL16 and viral capsids. It is also the first to show that UL16 assists the transport of other viral proteins to organelles. Previous researches on pUL16 usually emphasized its interaction with pUL11, pUL21, and gE, and sometimes commented on pUL49 and gD. Our research focuses on the novel interaction between pUL16 and VP26, thereby enriching the studies on herpesviruses and possibly providing different directions for researchers.<br /> (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Subjects :
- Active Transport, Cell Nucleus
Animals
Capsid Proteins genetics
Cell Line
Chlorocebus aethiops
Female
Gene Deletion
HEK293 Cells
Herpesvirus 1, Suid genetics
Humans
Mice, Inbred BALB C
Swine
Vero Cells
Virus Replication
Mice
Capsid Proteins metabolism
Cell Nucleus metabolism
Herpesvirus 1, Suid metabolism
Protein Interaction Domains and Motifs
Viral Proteins genetics
Viral Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2542
- Volume :
- 257
- Database :
- MEDLINE
- Journal :
- Veterinary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 33915344
- Full Text :
- https://doi.org/10.1016/j.vetmic.2021.109080