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Measurement of Genetic Mobility Using a Transposon-Based Marker System in Sorghum.

Authors :
Lyu JI
Jo YD
Ahn JW
Kim JB
Kwon SJ
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2250, pp. 195-205.
Publication Year :
2021

Abstract

Transposable elements (TEs) are ubiquitous repetitive components of eukaryotic organisms that show mobility in the genome against diverse stresses. TEs contribute considerably to the size, structure, and plasticity of genomes and also play an active role in genome evolution by helping their hosts adapt to novel conditions by conferring useful characteristics. We developed a simple and rapid method for investigation of genetic mobility and diversity among TEs in combination with a target region amplification polymorphism (TE-TRAP) marker system in gamma-irradiated sorghum mutants. The TE-TRAP marker system reveals a high level of genetic diversity, which provides a useful marker resource for genetic mobility research.

Details

Language :
English
ISSN :
1940-6029
Volume :
2250
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
33900606
Full Text :
https://doi.org/10.1007/978-1-0716-1134-0_19