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Measurement of Genetic Mobility Using a Transposon-Based Marker System in Sorghum.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2250, pp. 195-205. - Publication Year :
- 2021
-
Abstract
- Transposable elements (TEs) are ubiquitous repetitive components of eukaryotic organisms that show mobility in the genome against diverse stresses. TEs contribute considerably to the size, structure, and plasticity of genomes and also play an active role in genome evolution by helping their hosts adapt to novel conditions by conferring useful characteristics. We developed a simple and rapid method for investigation of genetic mobility and diversity among TEs in combination with a target region amplification polymorphism (TE-TRAP) marker system in gamma-irradiated sorghum mutants. The TE-TRAP marker system reveals a high level of genetic diversity, which provides a useful marker resource for genetic mobility research.
- Subjects :
- Amplified Fragment Length Polymorphism Analysis methods
DNA, Plant analysis
DNA, Plant genetics
Electrophoresis methods
Evolution, Molecular
Genome Size genetics
Polymerase Chain Reaction methods
Polymorphism, Genetic
DNA Transposable Elements genetics
Genetic Variation
Genome, Plant genetics
Sorghum genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2250
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 33900606
- Full Text :
- https://doi.org/10.1007/978-1-0716-1134-0_19