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Production of D-lactic acid containing polyhydroxyalkanoate polymers in yeast Saccharomyces cerevisiae.
- Source :
-
Journal of industrial microbiology & biotechnology [J Ind Microbiol Biotechnol] 2021 Jul 01; Vol. 48 (5-6). - Publication Year :
- 2021
-
Abstract
- Polyhydroxyalkanoates (PHAs) provide biodegradable and bio-based alternatives to conventional plastics. Incorporation of 2-hydroxy acid monomers into polymer, in addition to 3-hydroxy acids, offers possibility to tailor the polymer properties. In this study, poly(D-lactic acid) (PDLA) and copolymer P(LA-3HB) were produced and characterized for the first time in the yeast Saccharomyces cerevisiae. Expression of engineered PHA synthase PhaC1437Ps6-19, propionyl-CoA transferase Pct540Cp, acetyl-CoA acetyltransferase PhaA, and acetoacetyl-CoA reductase PhaB1 resulted in accumulation of 3.6% P(LA-3HB) and expression of engineered enzymes PhaC1Pre and PctMe resulted in accumulation of 0.73% PDLA of the cell dry weight (CDW). According to NMR, P(LA-3HB) contained D-lactic acid repeating sequences. For reference, expression of PhaA, PhaB1, and PHA synthase PhaC1 resulted in accumulation 11% poly(hydroxybutyrate) (PHB) of the CDW. Weight average molecular weights of these polymers were comparable to similar polymers produced by bacterial strains, 24.6, 6.3, and 1 130 kDa for P(LA-3HB), PDLA, and PHB, respectively. The results suggest that yeast, as a robust and acid tolerant industrial production organism, could be suitable for production of 2-hydroxy acid containing PHAs from sugars or from 2-hydroxy acid containing raw materials. Moreover, the wide substrate specificity of PHA synthase enzymes employed increases the possibilities for modifying copolymer properties in yeast in the future.<br /> (© The Author(s) 2021. Published by Oxford University Press on behalf of Society of Industrial Microbiology and Biotechnology.)
- Subjects :
- Acetyl-CoA C-Acetyltransferase genetics
Acetyl-CoA C-Acetyltransferase metabolism
Acyltransferases genetics
Acyltransferases metabolism
Alcohol Oxidoreductases genetics
Alcohol Oxidoreductases metabolism
Coenzyme A-Transferases genetics
Coenzyme A-Transferases metabolism
Escherichia coli metabolism
Genetic Engineering
Hydroxybutyrates metabolism
Industrial Microbiology
Metabolic Networks and Pathways
Polyhydroxyalkanoates chemistry
Lactic Acid metabolism
Polyhydroxyalkanoates biosynthesis
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1476-5535
- Volume :
- 48
- Issue :
- 5-6
- Database :
- MEDLINE
- Journal :
- Journal of industrial microbiology & biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 33899921
- Full Text :
- https://doi.org/10.1093/jimb/kuab028