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Campylobacter vulpis sp. nov. isolated from wild red foxes.

Authors :: Parisi A
Chiara M
Caffara M
Mion D
Miller WG
Caruso M
Manzari C
Florio D
Capozzi L
D'Erchia AM
Manzulli V
Zanoni RG
Source :: Systematic and applied microbiology [Syst Appl Microbiol] 2021 May; Vol. 44 (3), pp. 126204. Date of Electronic Publication: 2021 Apr 02.
Publication Year :: 2021
Abstract: During a sampling of wild red foxes (Vulpes vulpes) for the detection of Epsilonproteobacteria, 14 strains were isolated from the caecal contents of 14 epidemiologically-unrelated animals. A genus-specific PCR indicated that the isolates belonged to the genus Campylobacter. Based on the results of a species-specific PCR, the isolates were initially identified as C. upsaliensis. However, multi-locus sequence typing (MLST) revealed that the isolates were significantly different from the C. upsaliensis present in the MLST database. A polyphasic study, including conventional biochemical and tolerance characteristics, morphology by transmission electron microscopy (TEM), MALDI-TOF analysis, and genetic comparisons based on partial 16S rDNA and atpA gene sequences, was undertaken. Finally, the complete genome sequence of the type strain 251/13 <superscript>T</superscript> and the draft genome sequences of the other isolates were determined. Average nucleotide identity, average amino acid identity and in silico DNA-DNA hybridization analyses confirmed that the isolates represent a novel taxon for which the name Campylobacter vulpis sp. nov. is proposed, with isolate 251/13 <superscript>T</superscript> (=CCUG 70587 <superscript>T</superscript>  = LMG 30110 <superscript>T</superscript> ) as the type strain. In order to allow a rapid discrimination of C. vulpis from the closely-related C. upsaliensis, a specific PCR test was designed, based on atpA gene sequences.<br /> (Copyright © 2021 Elsevier GmbH. All rights reserved.)