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Simiao Wan attenuates monosodium urate crystal-induced arthritis in rats through contributing to macrophage M2 polarization.
- Source :
-
Journal of ethnopharmacology [J Ethnopharmacol] 2021 Jul 15; Vol. 275, pp. 114123. Date of Electronic Publication: 2021 Apr 22. - Publication Year :
- 2021
-
Abstract
- Ethnopharmacological Relevance: Simiao Wan (SMW) is a classical traditional Chinese medicine (TCM) prescription to empirically treat gouty arthritis (GA) in TCM clinical practice. However, the potential mechanisms of SMW on GA are not fully evaluated.<br />Aim of Study: The aim of this study is to investigate the role of macrophage polarization in the anti-GA activity of SMW.<br />Materials and Methods: Rats were intragastricly treated with SMW for consecutive 7 days. On day 6, monosodium urate (MSU) crystal-induced arthritis (MIA) in the ankle joint was prepared. Paw volume, gait score and histological score were measured. Levels of interleukin (IL)-1β and IL-10 in serum were detected by enzyme-linked immunosorbent assay. Expressions of inducible nitric oxide synthase (iNOS), arginase (Arg)-1, phosphorylated (p)-p65, inhibitor of nuclear factor (NF)-κB (IκB)α, p-signal transducer and transcription activator (STAT)3 and p-Janus kinase (JAK)2 in synovial tissues were determined by Western blot.<br />Results: The elevated paw volume, gait score and histological score in MIA rats were significantly decreased by SMW treatment. Meanwhile, SMW significantly decreased the IL-1β level and increased the IL-10 level in serum of MIA rats. Furthermore, SMW reduced the expressions of iNOS, p-p65 and enhanced the expressions of Arg-1, IκBα, p-STAT3 and p-JAK2 in synovial tissues of MIA rats.<br />Conclusions: The results suggest that SMW attenuates the inflammation in MIA rats through promoting macrophage M2 polarization.<br /> (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Anti-Inflammatory Agents chemistry
Antirheumatic Agents chemistry
Arginase metabolism
Arthritis, Experimental pathology
Drugs, Chinese Herbal chemistry
Edema chemically induced
Edema drug therapy
Edema pathology
Extremities pathology
Gait drug effects
I-kappa B Proteins metabolism
Inflammation chemically induced
Inflammation drug therapy
Inflammation pathology
Interleukin-10 metabolism
Interleukin-1beta metabolism
Janus Kinase 2 metabolism
Macrophage Activation drug effects
Macrophages metabolism
Male
Neoplasm Proteins metabolism
Nitric Oxide Synthase Type II metabolism
Nucleocytoplasmic Transport Proteins metabolism
Rats, Sprague-Dawley
STAT3 Transcription Factor metabolism
Synovial Membrane drug effects
Synovial Membrane metabolism
Uric Acid toxicity
Rats
Anti-Inflammatory Agents pharmacology
Anti-Inflammatory Agents therapeutic use
Antirheumatic Agents pharmacology
Antirheumatic Agents therapeutic use
Arthritis, Experimental prevention & control
Drugs, Chinese Herbal pharmacology
Drugs, Chinese Herbal therapeutic use
Macrophages drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7573
- Volume :
- 275
- Database :
- MEDLINE
- Journal :
- Journal of ethnopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 33894285
- Full Text :
- https://doi.org/10.1016/j.jep.2021.114123