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Identification of differentially expressed genes and signaling pathways in human conjunctiva and reproductive tract infected with Chlamydia trachomatis.
- Source :
-
Human genomics [Hum Genomics] 2021 Apr 19; Vol. 15 (1), pp. 22. Date of Electronic Publication: 2021 Apr 19. - Publication Year :
- 2021
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Abstract
- Background: Currently, Chlamydia trachomatis-specific host defense mechanisms in humans remain poorly defined. To study the characteristics of host cells infected early with Chlamydia trachomatis, we used bioinformatics methods to analyze the RNA transcription profiles of the conjunctiva, fallopian tubes, and endometrium in humans infected with Chlamydia trachomatis.<br />Method: The gene expression profiles of GSE20430, GSE20436, GSE26692, and GSE41075 were downloaded from the Gene Expression Synthesis (GEO) database. Then, we obtained the differentially expressed genes (DEGs) through the R 4.0.1 software. STRING was used to construct protein-protein interaction (PPI) networks; then, the Cytoscape 3.7.2 software was used to visualize the PPI and screen hub genes. GraphPad Prism 8.0 software was used to verify the expression of the hub gene. In addition, the gene-miRNA interaction was constructed on the NetworkAnalyst 3.0 platform using the miRTarBase v8.0 database.<br />Results: A total of 600 and 135 DEGs were screened out in the conjunctival infection group and the reproductive tract infection group, respectively. After constructing a PPI network and verifying the hub genes, CSF2, CD40, and CSF3 in the reproductive tract infection group proved to have considerable statistical significance.<br />Conclusion: In our research, the key genes in the biological process of reproductive tract infection with Chlamydia trachomatis were clarified through bioinformatics analysis. These hub genes may be further used in clinical treatment and clinical diagnosis.
- Subjects :
- Chlamydia trachomatis pathogenicity
Computational Biology
Conjunctiva microbiology
Conjunctiva parasitology
Fallopian Tubes metabolism
Fallopian Tubes microbiology
Fallopian Tubes pathology
Female
Gene Regulatory Networks genetics
Host-Pathogen Interactions genetics
Humans
MicroRNAs genetics
Protein Interaction Maps genetics
Reproductive Tract Infections microbiology
Reproductive Tract Infections pathology
Signal Transduction genetics
Software
CD40 Antigens genetics
Chlamydia trachomatis genetics
Conjunctiva metabolism
Granulocyte-Macrophage Colony-Stimulating Factor genetics
Reproductive Tract Infections genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1479-7364
- Volume :
- 15
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Human genomics
- Publication Type :
- Academic Journal
- Accession number :
- 33875006
- Full Text :
- https://doi.org/10.1186/s40246-021-00313-8