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Development and characterization of a new chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin for endodontic regeneration.

Authors :
Caballero-Flores H
Nabeshima CK
Sarra G
Moreira MS
Arana-Chavez VE
Marques MM
Machado MEL
Source :
Dental materials : official publication of the Academy of Dental Materials [Dent Mater] 2021 Jul; Vol. 37 (7), pp. e414-e425. Date of Electronic Publication: 2021 Apr 16.
Publication Year :
2021

Abstract

Objective: An ideal scaffold for endodontic regeneration should allow the predictableness of the new tissue organization and limit the negative impact of residual bacteria. Therefore, composition and functionalization of the scaffold play an important role in tissue bioengineering. The objective of this study was to assess the morphological, physicochemical, biological and antimicrobial properties of a new solid chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin.<br />Methods: Scaffolds based on chitosan (Ch); chitosan associated with gelatin and genipin (ChGG); and chitosan associated with gelatin, microparticulate dentin and genipin (ChGDG) were prepared by using the freeze-drying method. The morphology of the scaffolds was analyzed by scanning electron microscopy (SEM). The physicochemical properties were assessed for biodegradation, swelling and total released proteins. The biological aspects of the scaffolds were assessed using human cells from the apical papilla (hCAPs). Cell morphology and adhesion to the scaffolds were evaluated by SEM, cytotoxicity and cell proliferation by MTT reduction-assay. Cell differentiation in scaffolds was assessed by using alizarin red assay. The antimicrobial effect of the scaffolds was evaluated by using the bacterial culture method, and bacterial adhesion to the scaffolds was observed by SEM.<br />Results: All the scaffolds presented porous structures. The ChCDG had more protein release, adhesion, proliferation and differentiation of hCAPs, and bacteriostatic effect on Enterococcus faecalis than Ch and ChGG (p < 0.05).<br />Significance: The chitosan associated with gelatin, microparticulate dentin and genipin has morphological, physicochemical, biological and antibacterial characteristics suitable for their potential use as scaffold in regenerative endodontics.<br /> (Copyright © 2021 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1879-0097
Volume :
37
Issue :
7
Database :
MEDLINE
Journal :
Dental materials : official publication of the Academy of Dental Materials
Publication Type :
Academic Journal
Accession number :
33867170
Full Text :
https://doi.org/10.1016/j.dental.2021.03.016