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A novel plasmid from Aerococcus urinaeequi of porcine origin co-harboring the tetracycline resistance genes tet(58) and tet(61).
- Source :
-
Veterinary microbiology [Vet Microbiol] 2021 Jun; Vol. 257, pp. 109065. Date of Electronic Publication: 2021 Apr 09. - Publication Year :
- 2021
-
Abstract
- Tetracyclines are the broad-spectrum agents used in veterinary medicine and food animal production. Known mechanisms of tetracycline resistance include ribosome protection, active efflux and enzymatic inactivation. However, the presence of two different tet genes conferring different resistance mechanisms on the same plasmid has rarely been reported. In this study, we identified the tandem tetracycline resistance genes tet(61)-tet(58) on the novel plasmid pT4303. These tet genes were identified for the first time in Aerococcus urinaeequi. Reduced susceptibility to doxycycline was observed in S. aureus RN4220 harboring tet(61) when an extra tet(58) was expressed. Plasmid pT4303 was electrotransformed into S. aureus RN4220, E. faecalis JH2-2, S. suis BAA and E. coli DH5α and conferred tetracycline resistance (MIC ≥ 16) in both Gram-positive and Gram-negative bacteria, assuming that it might serve as a vehicle for the dissemination of the tetracycline resistance genes tet(61) and tet(58).<br /> (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Subjects :
- Aerococcus drug effects
Aerococcus isolation & purification
Animals
Anti-Bacterial Agents pharmacology
Genome, Bacterial
Gram-Negative Bacteria drug effects
Gram-Negative Bacteria genetics
Gram-Positive Bacteria drug effects
Gram-Positive Bacteria genetics
Microbial Sensitivity Tests
Plasmids isolation & purification
Swine
Tetracyclines pharmacology
Whole Genome Sequencing
Aerococcus genetics
Genes, Bacterial
Plasmids genetics
Tetracycline Resistance genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2542
- Volume :
- 257
- Database :
- MEDLINE
- Journal :
- Veterinary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 33866063
- Full Text :
- https://doi.org/10.1016/j.vetmic.2021.109065