Back to Search
Start Over
Quantification of trivalent non-replicating rotavirus vaccine antigens in the presence of aluminum adjuvant.
- Source :
-
Journal of immunological methods [J Immunol Methods] 2021 Jul; Vol. 494, pp. 113056. Date of Electronic Publication: 2021 Apr 13. - Publication Year :
- 2021
-
Abstract
- Parenterally administered rotavirus vaccines may overcome the low efficacy observed in resource-poor regions that use live oral formulations. We have reported work on a trivalent nonreplicating rotavirus vaccine (NRRV) for parenteral administration consisting of the recombinant tetanus toxoid P2 CD4 epitope fused to a truncated VP8* fragment (P2-VP8*) for the P[4], P[6], and P[8] serotypes of rotavirus adjuvanted with aluminum. An essential part of developing this vaccine candidate was devising quantification methods for each antigen in the trivalent NRRV in the presence of aluminum adjuvant. This report describes the development of quantitative inhibition enzyme-linked immunosorbent assays (ELISAs) for in vitro antigenicity determination of the adjuvanted trivalent NRRV using serotype-specific monoclonal antibodies (mAbs) against each of the P2-VP8* antigens. Adjuvanted trivalent vaccine samples are titrated and incubated with a constant concentration of specific mAbs against each NRRV P2-VP8* antigen variant. Unbound mAbs are measured by ELISA to indirectly quantify the amount of each antigen present in the trivalent vaccine. Sensitive, specific, and reproducible inhibition ELISAs were developed and qualified for each antigen and used for final product quantification and release testing without desorption of the vaccine antigen.<br /> (Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Adjuvants, Immunologic
Antibodies, Monoclonal metabolism
Humans
Immunogenicity, Vaccine
Infusions, Parenteral
Rotavirus Vaccines genetics
Enzyme-Linked Immunosorbent Assay methods
Epitopes, T-Lymphocyte genetics
Peptides genetics
RNA-Binding Proteins genetics
Recombinant Fusion Proteins genetics
Rotavirus Vaccines immunology
Tetanus Toxoid genetics
Viral Nonstructural Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7905
- Volume :
- 494
- Database :
- MEDLINE
- Journal :
- Journal of immunological methods
- Publication Type :
- Academic Journal
- Accession number :
- 33857473
- Full Text :
- https://doi.org/10.1016/j.jim.2021.113056