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The CD36 Ligand-Promoted Autophagy Protects Retinal Pigment Epithelial Cells from Oxidative Stress.
- Source :
-
Oxidative medicine and cellular longevity [Oxid Med Cell Longev] 2021 Mar 28; Vol. 2021, pp. 6691402. Date of Electronic Publication: 2021 Mar 28 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- The retinal pigment epithelium (RPE) performs many functions that maintain photoreceptor health. Oxidative damage to the RPE is a critical component in the pathogenesis of eye diseases such as age-related macular degeneration (AMD). Ligands of the cluster of differentiation 36 (CD36) have previously preserved photoreceptor integrity in mouse models of AMD. The cytoprotective effect of the CD36 ligand MPE-001 on RPE cells has now been elucidated employing a model of oxidative stress. Sodium iodate (NaIO <subscript>3</subscript> ) induced formation of reactive oxygen species and apoptosis in human RPE cells, which were decreased by MPE-001 without affecting antioxidant enzyme transcription. Immunoblotting and immunostaining assays showed a restorative effect of MPE-001 on the autophagic flux disrupted by NaIO <subscript>3</subscript> , which was associated with an increase in syntaxin 17-positive mature autophagosomes. The cytoprotective effect of MPE-001 was completely abolished by the autophagy inhibitors wortmannin and bafilomycin A1. In conclusion, we report for the first time an autophagy-dependent protection of RPE cells from oxidative stress by a CD36 ligand.<br />Competing Interests: The authors declare that there is no conflict of interest regarding the publication of this paper.<br /> (Copyright © 2021 Marie-France Dorion et al.)
- Subjects :
- Autophagy drug effects
Cell Line
Humans
Iodates pharmacology
Ligands
Molecular Targeted Therapy
Oligopeptides chemistry
Reactive Oxygen Species metabolism
Retinal Pigment Epithelium drug effects
CD36 Antigens metabolism
Oligopeptides pharmacology
Oxidative Stress drug effects
Retinal Pigment Epithelium metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1942-0994
- Volume :
- 2021
- Database :
- MEDLINE
- Journal :
- Oxidative medicine and cellular longevity
- Publication Type :
- Academic Journal
- Accession number :
- 33854697
- Full Text :
- https://doi.org/10.1155/2021/6691402