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LGR6 marks nephron progenitor cells.
- Source :
-
Developmental dynamics : an official publication of the American Association of Anatomists [Dev Dyn] 2021 Nov; Vol. 250 (11), pp. 1568-1583. Date of Electronic Publication: 2021 May 06. - Publication Year :
- 2021
-
Abstract
- Background: Nephron progenitor cells (NPCs) undergo a stepwise process to generate all mature nephron structures. Mesenchymal to epithelial transition (MET) is considered a multistep process of NPC differentiation to ensure progressive establishment of new nephrons. However, despite this important role, to date, no marker for NPCs undergoing MET in the nephron exists.<br />Results: Here, we identify LGR6 as a NPC marker, expressed in very early cap mesenchyme, pre-tubular aggregates, renal vesicles, and in segments of S-shaped bodies, following the trajectory of MET. By using a lineage tracing approach in embryonic explants in combination with confocal imaging and single-cell RNA sequencing, we provide evidence for the multiple fates of LGR6+ cells during embryonic nephrogenesis. Moreover, by using long-term in vivo lineage tracing, we show that postnatal LGR6+ cells are capable of generating the multiple lineages of the nephrons.<br />Conclusions: Given the profound early mesenchymal expression and MET signature of LGR6 <superscript>+</superscript> cells, together with the lineage tracing of mesenchymal LGR6 <superscript>+</superscript> cells, we conclude that LGR6+ cells contribute to all nephrogenic segments by undergoing MET. LGR6+ cells can therefore be considered an early committed NPC population during embryonic and postnatal nephrogenesis with potential regenerative capability.<br /> (© 2021 The Authors. Developmental Dynamics published by Wiley Periodicals LLC on behalf of American Association of Anatomists.)
- Subjects :
- Cell Differentiation
Mesoderm
Organogenesis genetics
Nephrons
Stem Cells
Subjects
Details
- Language :
- English
- ISSN :
- 1097-0177
- Volume :
- 250
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Developmental dynamics : an official publication of the American Association of Anatomists
- Publication Type :
- Academic Journal
- Accession number :
- 33848015
- Full Text :
- https://doi.org/10.1002/dvdy.346