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VESPER: global and local cryo-EM map alignment using local density vectors.

Authors :
Han X
Terashi G
Christoffer C
Chen S
Kihara D
Source :
Nature communications [Nat Commun] 2021 Apr 07; Vol. 12 (1), pp. 2090. Date of Electronic Publication: 2021 Apr 07.
Publication Year :
2021

Abstract

An increasing number of density maps of biological macromolecules have been determined by cryo-electron microscopy (cryo-EM) and stored in the public database, EMDB. To interpret the structural information contained in EM density maps, alignment of maps is an essential step for structure modeling, comparison of maps, and for database search. Here, we developed VESPER, which captures the similarity of underlying molecular structures embedded in density maps by taking local gradient directions into consideration. Compared to existing methods, VESPER achieved substantially more accurate global and local alignment of maps as well as database retrieval.

Details

Language :
English
ISSN :
2041-1723
Volume :
12
Issue :
1
Database :
MEDLINE
Journal :
Nature communications
Publication Type :
Academic Journal
Accession number :
33828103
Full Text :
https://doi.org/10.1038/s41467-021-22401-y