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MiR-155 Inhibits Malignant Biological Behavior of Human Liver Cancer Cells by Regulating SRPK1.
- Source :
-
Technology in cancer research & treatment [Technol Cancer Res Treat] 2021 Jan-Dec; Vol. 20, pp. 1533033820957021. - Publication Year :
- 2021
-
Abstract
- Although the treatment of liver cancer has made great progress, the mechanism of its occurrence is not completely clear. miR-155 plays an important regulatory role in tumorigenesis and development, including survival, proliferation, migration and invasion. However, the role and regulatory mechanism of miR-155 in liver cancer has rarely been reported. We analyzed miR-155 expression in liver cancer tissue samples and cell lines by qRT-PCR. The expression of miR-155 was measured by qRT-PCR before and after miR-155-mimic and sh-miR-155 transfection. CCK-8 and clonogenic assays were used to detect the proliferation of liver cancer cells. Cell scratch and invasion assays were used to detect migration and invasion. RNA-seq was used to detect the difference in RNA expression in liver cancer cells. SRPK1 expression was detected in liver cancer cells before and after transfection by qRT-PCR and western blotting. We observed that miR-155 was downregulated in liver cancer tissues compared with normal tissues. Furthermore, we demonstrated that liver cancer cell proliferation, migration and invasion are markedly suppressed by miR-155. Importantly, we also demonstrated that SRPK1 is directly regulated by miR-155 during the process of liver cancer cell proliferation and metastasis. Finally, the overexpression of miR-155 inhibits malignant biological behavior of human liver cancer cells. We report the abnormal expression of the miR-155 cluster in liver cancer cells, which inhibits cancer cell proliferation and metastasis. In addition, we identified SRPK1 as a target gene of miR-155 during the process of liver cancer cell proliferation and metastasis.
- Subjects :
- Carcinoma, Hepatocellular metabolism
Carcinoma, Hepatocellular secondary
Cell Movement genetics
Cell Proliferation genetics
Down-Regulation
Female
Gene Expression
Hep G2 Cells
Humans
Liver metabolism
Liver Neoplasms metabolism
Liver Neoplasms pathology
Male
Middle Aged
Neoplasm Metastasis genetics
Protein Serine-Threonine Kinases metabolism
Sequence Analysis, RNA
Transcriptome
Transfection
Tumor Stem Cell Assay
Carcinoma, Hepatocellular genetics
Liver Neoplasms genetics
MicroRNAs genetics
Protein Serine-Threonine Kinases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1533-0338
- Volume :
- 20
- Database :
- MEDLINE
- Journal :
- Technology in cancer research & treatment
- Publication Type :
- Academic Journal
- Accession number :
- 33827350
- Full Text :
- https://doi.org/10.1177/1533033820957021