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Naoxintong capsules modulates tumor necrosis factor--induced endothelial senescence through silent information regulator 1 signaling.
- Source :
-
Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan [J Tradit Chin Med] 2021 Apr; Vol. 41 (2), pp. 212-218. - Publication Year :
- 2021
-
Abstract
- Objective: To investigate the protective effects of Naoxintong capsules ( NXT)on tumor necrosis factor-α (TNF-α) -induced senescence inendothelial cells and its mechanism.<br />Methods: Human umbilical vascular endothelial cells (HUVECs) were treated with TNF-α ± NXT and assessed for silent information regulator 1 (SIRT1) expression and signaling. Cells were stained with beta-galactosidase to assess the levels of cellular senescence. SIRT1 was silenced through siRNA transfection.<br />Results: TNF-α treatment led to the downregulation of SIRT1, resulting in forkhead box O1 (FoxO-1) acetylation, p53 acetylation and enhanced p21 expression. Following TNF-α treatment, higher SA β-Gal activity improved. TNF-α enhanced the migration of HUVECs and increased SIRT1 expression, both of which were attenuated by NXT treatment. The downstream targets of SIRT1 including FoxO-1/p53/p21 were also modulated, and HUVECs were protected from TNF-α-induced senescence. In contrast, the NXT-mediated protection was prevented by SIRT1 silencing.<br />Conclusions: These findings suggest that sustained endothelial senescence can be induced by TNF-α stimulation via the SIRT1/FoxO-1/p53/p21 pathway. The protection of NXT against TNF- was partially mediated through its effects on SIRT1. This highlights the promise of NXT as a therapeutic for atherosclerosis.
- Subjects :
- Capsules pharmacology
Cells, Cultured
Cellular Senescence drug effects
Down-Regulation drug effects
Human Umbilical Vein Endothelial Cells metabolism
Humans
Signal Transduction drug effects
Sirtuin 1 genetics
Tumor Necrosis Factor-alpha genetics
Drugs, Chinese Herbal pharmacology
Human Umbilical Vein Endothelial Cells cytology
Human Umbilical Vein Endothelial Cells drug effects
Sirtuin 1 metabolism
Tumor Necrosis Factor-alpha metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2589-451X
- Volume :
- 41
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan
- Publication Type :
- Academic Journal
- Accession number :
- 33825400