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Quantitative detection of circulating MT-ND1 as a potential biomarker for colorectal cancer.
- Source :
-
Bosnian journal of basic medical sciences [Bosn J Basic Med Sci] 2021 Oct 01; Vol. 21 (5), pp. 577-586. Date of Electronic Publication: 2021 Oct 01. - Publication Year :
- 2021
-
Abstract
- Liquid biopsy represents a diagnostic and monitoring tool and the circulating cell-free mitochondrial DNA (mtDNA) plays a vital role in tumor diagnosis and dynamic assessment. Colorectal cancer (CRC) is one of the most common fatal cancers worldwide. Mitochondrially encoded NADH dehydrogenase subunit 1 (MT-ND1) encodes the biggest subunit of respiratory complex I of mtDNA, and mutations in the MT-ND1 are common in CRC. We sought to determine if mutations in circulating MT-ND1 could be a potential biomarker for colorectal cancer. In this study, twenty-two CRC patients at Zhujiang Hospital were included. We mainly used droplet digital PCR to determine the mutation status of MT-ND1, combined with clinical data. In the experiment in vivo, cell-free mtDNA generally presented high concordance with tumor tissues. By quantitative PCR, the MT-ND1 content of plasma in CRC patients was significantly higher than that in healthy individuals (58.01 vs. 0.64, p=0.027). The detection of circulating MT-ND1 content and variants (m.3606 A>G, m.3970 C>T, m.4071 C>T, m.4086 C>T) in cfDNA showed a good correlation with predicted tumor response and progression to chemotherapy. In conclusion, the content and variants of circulating MT-ND1 may become a versatile tool for the diagnosis and monitoring of colorectal cancer.
- Subjects :
- Cell Line, Tumor
Colorectal Neoplasms pathology
Colorectal Neoplasms therapy
DNA, Mitochondrial blood
Germ-Line Mutation
Humans
Liquid Biopsy
Neoplasm Staging
Prognosis
Biomarkers, Tumor blood
Colorectal Neoplasms blood
Colorectal Neoplasms genetics
NADH Dehydrogenase blood
NADH Dehydrogenase genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1840-4812
- Volume :
- 21
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Bosnian journal of basic medical sciences
- Publication Type :
- Academic Journal
- Accession number :
- 33823124
- Full Text :
- https://doi.org/10.17305/bjbms.2021.5576