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MiR-193a-3p Promotes Fracture Healing via Targeting PTEN Gene.
- Source :
-
Molecular biotechnology [Mol Biotechnol] 2021 Jul; Vol. 63 (7), pp. 605-612. Date of Electronic Publication: 2021 Apr 04. - Publication Year :
- 2021
-
Abstract
- The aim of this study was to investigate the role and potential mechanism of miR-193a-3p in fracture healing. The 70 fragility fracture patients and 45 healthy controls were enrolled in this study. Quantitative real-time PCR (qRT-PCR) was used for the measurement of the expression levels of miR-193a-3p and PTEN. MTT assay and flow cytometry were used to detect cell viability and apoptosis in the mouse osteoblastic cell line MC3T3-E1. Luciferase reporter assay was performed to confirm the correlation of miR-193a-3p with PTEN. The serum expression level of miR-193a-3p showed no significant change in fracture patients 7 days after fixation treatment, but over time, there was a significant decrease in the expression at 14 days and 21 days after treatment (P < 0.01). Overexpression of miR-193a-3p significantly enhanced cell viability and inhibited cell apoptosis in MC3T3-E1 cells (P < 0.001). Serum PTEN level in fracture patients was increased gradually during the fracture healing process (P < 0.01). PTEN was demonstrated to be a target gene of miR-9-5p and reversed the effect of miR-193a-3p on cell viability and apoptosis (P < 0.001). miR-193a-3p promoted fracture healing via regulating PTEN and may serve as a novel potential target for enhancing bone repair of fragility fracture.
- Subjects :
- Animals
Case-Control Studies
Cell Line
Cell Movement
Cell Proliferation
Cell Survival
Fractures, Bone blood
Fractures, Bone genetics
Gene Expression Regulation, Neoplastic
Humans
Mice
MicroRNAs blood
Orthopedic Procedures
PTEN Phosphohydrolase blood
Up-Regulation
Fracture Healing
Fractures, Bone surgery
MicroRNAs genetics
PTEN Phosphohydrolase genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1559-0305
- Volume :
- 63
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Molecular biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 33813678
- Full Text :
- https://doi.org/10.1007/s12033-021-00322-x