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KLF4, Slug and EMT in Head and Neck Squamous Cell Carcinoma.
- Source :
-
Cells [Cells] 2021 Mar 03; Vol. 10 (3). Date of Electronic Publication: 2021 Mar 03. - Publication Year :
- 2021
-
Abstract
- Epithelial to mesenchymal transition (EMT) is clinically relevant in head and neck squamous cell carcinoma (HNSCC). We hypothesized that EMT-transcription factors (EMT-TFs) and an anti-EMT factor, Krüppel-like-factor-4 (KLF4) regulate EMT in HNSCC. Ten control mucosa and 37 HNSCC tissue samples and three HNSCC cell lines were included for investigation of EMT-TFs, KLF4 and vimentin at mRNA and protein levels. Slug gene expression was significantly higher, whereas, KLF4 gene expression was significantly lower in HNSCC than in normal mucosa. In the majority of HNSCC samples, there was a significant negative correlation between KLF4 and Slug gene expression. Slug gene expression was significantly higher in human papilloma virus (HPV) negative HNSCC, and in tumor samples with irregular p53 gene sequence. Transforming-growth-factor-beta-1 (TGF- β1) contributed to downregulation of KLF4 and upregulation of Slug. Two possible regulatory pathways could be suggested: (1) EMT-factors induced pathway, where TGF-β1 induced Slug together with vimentin, and KLF4 was down regulated at the same time; (2) p53 mutations contributed to upregulation and stabilization of Slug, where also KLF4 could co-exist with EMT-TFs.
- Subjects :
- Aged
Aged, 80 and over
Case-Control Studies
Cell Line, Tumor
Cell Movement
Cell Proliferation
Epithelial-Mesenchymal Transition genetics
Female
Gene Expression Regulation, Neoplastic
Head and Neck Neoplasms metabolism
Head and Neck Neoplasms mortality
Head and Neck Neoplasms pathology
Humans
Kruppel-Like Factor 4
Kruppel-Like Transcription Factors metabolism
Male
Middle Aged
Neoplasm Staging
Nuclear Proteins metabolism
Signal Transduction
Snail Family Transcription Factors metabolism
Squamous Cell Carcinoma of Head and Neck metabolism
Squamous Cell Carcinoma of Head and Neck mortality
Squamous Cell Carcinoma of Head and Neck pathology
Survival Analysis
Transforming Growth Factor beta1 genetics
Transforming Growth Factor beta1 metabolism
Tumor Suppressor Protein p53 metabolism
Twist-Related Protein 1 metabolism
Vimentin genetics
Vimentin metabolism
Zinc Finger E-box-Binding Homeobox 1 genetics
Zinc Finger E-box-Binding Homeobox 1 metabolism
Head and Neck Neoplasms genetics
Kruppel-Like Transcription Factors genetics
Nuclear Proteins genetics
Snail Family Transcription Factors genetics
Squamous Cell Carcinoma of Head and Neck genetics
Tumor Suppressor Protein p53 genetics
Twist-Related Protein 1 genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2073-4409
- Volume :
- 10
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Cells
- Publication Type :
- Academic Journal
- Accession number :
- 33802627
- Full Text :
- https://doi.org/10.3390/cells10030539