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Molecular Epidemiology of Novirhabdoviruses Emerging in Iranian Trout Farms.
- Source :
-
Viruses [Viruses] 2021 Mar 10; Vol. 13 (3). Date of Electronic Publication: 2021 Mar 10. - Publication Year :
- 2021
-
Abstract
- Novirhabdoviruses cause large epizootics and economic losses of farmed trout. In this study, we surveyed Viral hemorrhagic septicemia virus and Infectious hematopoietic and necrosis virus (VHSV and IHNV) through both monitoring and investigation of clinical outbreaks reported by farmers in the regions with major rainbow trout production in Iran from 2015 to 2019. RT-PCR assays of the kidney samples and cell culture (EPC/FHM cells) samples confirmed the presence of the viruses, with 9 VHSV and 4 IHNV isolates, in both endemic and new areas of Iran. Sequence analysis of the G gene revealed that VHSV isolates belonged to genogroup Ia, and IHNV isolates were clustered into genogroup E, both typical for isolates from European countries. A haplotype analysis based on non-homologous amino acids of the G gene supports the emergence of two lineages of IHNV from clade 1 (E-1), as well as VHSV clade 2 (Ia-2) of the European genogroups, confirming that VHSV and IHNV isolates in Iran, have originated from Europe possibly via imported eggs.
- Subjects :
- Animals
Base Sequence
Disease Outbreaks
Europe epidemiology
Fish Diseases virology
Fisheries
Genotype
Haplotypes genetics
Infectious hematopoietic necrosis virus genetics
Iran epidemiology
Molecular Epidemiology
Novirhabdovirus genetics
Novirhabdovirus isolation & purification
Phylogeny
Sequence Analysis, DNA
Fish Diseases epidemiology
Infectious hematopoietic necrosis virus isolation & purification
Oncorhynchus mykiss virology
Rhabdoviridae Infections epidemiology
Rhabdoviridae Infections veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1999-4915
- Volume :
- 13
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Viruses
- Publication Type :
- Academic Journal
- Accession number :
- 33802100
- Full Text :
- https://doi.org/10.3390/v13030448