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Long-Term Tracking and Dynamically Quantifying of Reversible Changes of Extracellular Ca 2+ in Multiple Brain Regions of Freely Moving Animals.
- Source :
-
Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2021 Jun 21; Vol. 60 (26), pp. 14429-14437. Date of Electronic Publication: 2021 Apr 28. - Publication Year :
- 2021
-
Abstract
- Understanding physiological and pathological processes in the brain requires tracking the reversible changes in chemical signals with long-term stability. We developed a new anti-biofouling microfiber array to real-time quantify extracellular Ca <superscript>2+</superscript> concentrations together with neuron activity across many regions in the mammalian brain for 60 days, in which the signal degradation was < ca. 8 %. The microarray with high tempo-spatial resolution (ca. 10 μm, ca. 1.3 s) was implanted into 7 brain regions of free-moving mice to monitor reversible changes of extracellular Ca <superscript>2+</superscript> upon ischemia-reperfusion processes. The changing sequence and rate of Ca <superscript>2+</superscript> in 7 brain regions were different during the stroke. ROS scavenger could protect Ca <superscript>2+</superscript> influx and neuronal activity after stroke, suggesting the significant influence of ROS on Ca <superscript>2+</superscript> overload and neuron death. We demonstrated this microarray is a versatile tool for investigating brain dynamic during pathological processes and drug treatment.<br /> (© 2021 Wiley-VCH GmbH.)
Details
- Language :
- English
- ISSN :
- 1521-3773
- Volume :
- 60
- Issue :
- 26
- Database :
- MEDLINE
- Journal :
- Angewandte Chemie (International ed. in English)
- Publication Type :
- Academic Journal
- Accession number :
- 33797152
- Full Text :
- https://doi.org/10.1002/anie.202102833