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Independence of chromatin conformation and gene regulation during Drosophila dorsoventral patterning.
- Source :
-
Nature genetics [Nat Genet] 2021 Apr; Vol. 53 (4), pp. 487-499. Date of Electronic Publication: 2021 Apr 01. - Publication Year :
- 2021
-
Abstract
- The relationship between chromatin organization and gene regulation remains unclear. While disruption of chromatin domains and domain boundaries can lead to misexpression of developmental genes, acute depletion of regulators of genome organization has a relatively small effect on gene expression. It is therefore uncertain whether gene expression and chromatin state drive chromatin organization or whether changes in chromatin organization facilitate cell-type-specific activation of gene expression. Here, using the dorsoventral patterning of the Drosophila melanogaster embryo as a model system, we provide evidence for the independence of chromatin organization and dorsoventral gene expression. We define tissue-specific enhancers and link them to expression patterns using single-cell RNA-seq. Surprisingly, despite tissue-specific chromatin states and gene expression, chromatin organization is largely maintained across tissues. Our results indicate that tissue-specific chromatin conformation is not necessary for tissue-specific gene expression but rather acts as a scaffold facilitating gene expression when enhancers become active.
- Subjects :
- Animals
Animals, Genetically Modified
Cell Differentiation
Chromatin metabolism
Drosophila Proteins metabolism
Drosophila melanogaster cytology
Drosophila melanogaster growth & development
Drosophila melanogaster metabolism
Embryo, Nonmammalian
Enhancer Elements, Genetic
Female
Genome
High-Throughput Nucleotide Sequencing
Histones genetics
Histones metabolism
Male
Organ Specificity
Promoter Regions, Genetic
Single-Cell Analysis
Transcription, Genetic
Body Patterning genetics
Cell Lineage genetics
Chromatin chemistry
Drosophila Proteins genetics
Drosophila melanogaster genetics
Gene Expression Regulation, Developmental
Subjects
Details
- Language :
- English
- ISSN :
- 1546-1718
- Volume :
- 53
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Nature genetics
- Publication Type :
- Academic Journal
- Accession number :
- 33795866
- Full Text :
- https://doi.org/10.1038/s41588-021-00799-x