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High-fidelity structured illumination microscopy by point-spread-function engineering.
- Source :
-
Light, science & applications [Light Sci Appl] 2021 Apr 01; Vol. 10 (1), pp. 70. Date of Electronic Publication: 2021 Apr 01. - Publication Year :
- 2021
-
Abstract
- Structured illumination microscopy (SIM) has become a widely used tool for insight into biomedical challenges due to its rapid, long-term, and super-resolution (SR) imaging. However, artifacts that often appear in SIM images have long brought into question its fidelity, and might cause misinterpretation of biological structures. We present HiFi-SIM, a high-fidelity SIM reconstruction algorithm, by engineering the effective point spread function (PSF) into an ideal form. HiFi-SIM can effectively reduce commonly seen artifacts without loss of fine structures and improve the axial sectioning for samples with strong background. In particular, HiFi-SIM is not sensitive to the commonly used PSF and reconstruction parameters; hence, it lowers the requirements for dedicated PSF calibration and complicated parameter adjustment, thus promoting SIM as a daily imaging tool.
Details
- Language :
- English
- ISSN :
- 2047-7538
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Light, science & applications
- Publication Type :
- Academic Journal
- Accession number :
- 33795640
- Full Text :
- https://doi.org/10.1038/s41377-021-00513-w