Back to Search
Start Over
Analysis of a photosynthetic cyanobacterium rich in internal membrane systems via gradient profiling by sequencing (Grad-seq).
- Source :
-
The Plant cell [Plant Cell] 2021 Apr 17; Vol. 33 (2), pp. 248-269. - Publication Year :
- 2021
-
Abstract
- Although regulatory small RNAs have been reported in photosynthetic cyanobacteria, the lack of clear RNA chaperones involved in their regulation poses a conundrum. Here, we analyzed the full complement of cellular RNAs and proteins using gradient profiling by sequencing (Grad-seq) in Synechocystis 6803. Complexes with overlapping subunits such as the CpcG1-type versus the CpcL-type phycobilisomes or the PsaK1 versus PsaK2 photosystem I pre(complexes) could be distinguished, supporting the high quality of this approach. Clustering of the in-gradient distribution profiles followed by several additional criteria yielded a short list of potential RNA chaperones that include an YlxR homolog and a cyanobacterial homolog of the KhpA/B complex. The data suggest previously undetected complexes between accessory proteins and CRISPR-Cas systems, such as a Csx1-Csm6 ribonucleolytic defense complex. Moreover, the exclusive association of either RpoZ or 6S RNA with the core RNA polymerase complex and the existence of a reservoir of inactive sigma-antisigma complexes is suggested. The Synechocystis Grad-seq resource is available online at https://sunshine.biologie.uni-freiburg.de/GradSeqExplorer/ providing a comprehensive resource for the functional assignment of RNA-protein complexes and multisubunit protein complexes in a photosynthetic organism.<br /> (© The Author(s) 2020. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
- Subjects :
- Bacterial Proteins metabolism
Gene Expression Regulation, Bacterial
Multiprotein Complexes metabolism
Phylogeny
Protein Binding
Protein Biosynthesis
Proteome metabolism
RNA, Bacterial genetics
RNA, Bacterial metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
Ribonucleoproteins metabolism
Thylakoids metabolism
Toxin-Antitoxin Systems
Transcriptome genetics
Intracellular Membranes metabolism
Photosynthesis genetics
Sequence Analysis, RNA
Synechocystis genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1532-298X
- Volume :
- 33
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Plant cell
- Publication Type :
- Academic Journal
- Accession number :
- 33793824
- Full Text :
- https://doi.org/10.1093/plcell/koaa017