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Heparin-mediated antibiotic delivery from an electrochemically-aligned collagen sheet.
- Source :
-
Bio-medical materials and engineering [Biomed Mater Eng] 2021; Vol. 32 (3), pp. 159-170. - Publication Year :
- 2021
-
Abstract
- Background: Implantable medical devices and hardware are prolific in medicine, but hardware associated infections remain a major issue.<br />Objective: To develop and evaluate a novel, biologic antimicrobial coating for medical implants.<br />Methods: Electrochemically compacted collagen sheets with and without crosslinked heparin were synthesized per a protocol developed by our group. Sheets were incubated in antibiotic solution (gentamicin or moxifloxacin) overnight, and in vitro activity was assessed with five-day diffusion assays against Pseudomonas aeruginosa. Antibiotic release over time from gentamicin-infused sheets was determined using in vitro elution and high performance liquid chromatography (HPLC).<br />Results: Collagen-heparin-antibiotic sheets demonstrated larger growth inhibition zones against P. aeruginosa compared to collagen-antibiotic alone sheets. This activity persisted for five days and was not impacted by rinsing sheets prior to evaluation. Rinsed collagen-antibiotic sheets did not produce any inhibition zones. Elution of gentamicin from collagen-heparin-gentamicin sheets was gradual and remained above the minimal inhibitory concentration for gentamicin-sensitive organisms for 29 days. Conversely, collagen-gentamicin sheets eluted their antibiotic load within 24 hours. Overall, heparin-associated sheets demonstrated larger inhibition zones against P. aeruginosa and prolonged elution profile via HPLC.<br />Conclusion: We developed a novel, local antibiotic delivery system that could be used to coat medical implants/hardware in the future and reduce post-operative infections.
- Subjects :
- Anti-Bacterial Agents
Collagen
Gentamicins
Pseudomonas aeruginosa
Heparin
Subjects
Details
- Language :
- English
- ISSN :
- 1878-3619
- Volume :
- 32
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Bio-medical materials and engineering
- Publication Type :
- Academic Journal
- Accession number :
- 33780355
- Full Text :
- https://doi.org/10.3233/BME-201133