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Genomic characteristics of mcr-1 and bla CTX-M-type in a single multidrug-resistant Escherichia coli ST93 from chicken in China.
- Source :
-
Poultry science [Poult Sci] 2021 May; Vol. 100 (5), pp. 101074. Date of Electronic Publication: 2021 Feb 27. - Publication Year :
- 2021
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Abstract
- This study was undertaken to discern the transmission characteristics of mcr-1 and bla <subscript>CTX-M-type</subscript> in one multidrug-resistant Escherichia coli LWY24 from chicken in China. The genetic profiles of LWY24 isolate were determined by conjugation, S1-pulsed-field gel electrophoresis, southern blot hybridization, and whole genome sequencing analysis. Meanwhile, co-transfer of plasmids in LWY24 isolate was screened by dual conjugation assays. The LWY24 isolate was identified as ST93, and harbored 3 conjugative plasmids, pLWY24J-3 (bla <subscript>CTX-M-55</subscript> -bearing IncFⅡ), pLWY24J-mcr-1 (mcr-1-carrying IncI2), and pLWY24J-4 (non-resistance-conferring IncI1), and one nonconjugative plasmid pLWY24 (bla <subscript>CTX-M-14</subscript> -containing IncHI2/IncHI2A). Numerous resistance genes, insertion sequences (especially IS26), and transposons were found in the 4 plasmids, suggesting that horizontal transmission have occurred by plasmid mating, homologous recombination, and transpositions. Under the selection pressure of cefotaxime and colistin or cefotaxime alone, the mcr-1-bearing plasmid and the bla <subscript>CTX-M-55</subscript> -harboring plasmid could be co-transferred at a similar frequency, with 8.00 × 10 <superscript>-4</superscript> or 9.00 × 10 <superscript>-4</superscript> transconjugants per donor cell, respectively. The specific shufflon region in mcr-1-encoding plasmid could generate up to 6 diverse PilV structures, which may further accelerate the horizontal transfer of plasmid. In conclusion, the transmission characteristics of mcr-1 and bla <subscript>CTX-M-type</subscript> in LWY24 isolate could due to clonal spread of ST93, selective pressure of cefotaxime, IS26-mediate homologous recombination and transposition, and the specific shufflon region.<br /> (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1525-3171
- Volume :
- 100
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Poultry science
- Publication Type :
- Academic Journal
- Accession number :
- 33774373
- Full Text :
- https://doi.org/10.1016/j.psj.2021.101074