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The Scf/Kit pathway implements self-organized epithelial patterning.
- Source :
-
Developmental cell [Dev Cell] 2021 Mar 22; Vol. 56 (6), pp. 795-810.e7. - Publication Year :
- 2021
-
Abstract
- How global patterns emerge from individual cell behaviors is poorly understood. In the Xenopus embryonic epidermis, multiciliated cells (MCCs) are born in a random pattern within an inner mesenchymal layer and subsequently intercalate at regular intervals into an outer epithelial layer. Using video microscopy and mathematical modeling, we found that regular pattern emergence involves mutual repulsion among motile immature MCCs and affinity toward outer-layer intercellular junctions. Consistently, Arp2/3-mediated actin remodeling is required for MCC patterning. Mechanistically, we show that the Kit tyrosine kinase receptor, expressed in MCCs, and its ligand Scf, expressed in outer-layer cells, are both required for regular MCC distribution. Membrane-associated Scf behaves as a potent adhesive cue for MCCs, while its soluble form promotes their mutual repulsion. Finally, Kit expression is sufficient to confer order to a disordered heterologous cell population. This work reveals how a single signaling system can implement self-organized large-scale patterning.<br />Competing Interests: Declaration of interests The authors declare no competing interests.<br /> (Copyright © 2021 Elsevier Inc. All rights reserved.)
- Subjects :
- Actin-Related Protein 2-3 Complex genetics
Actins metabolism
Animals
Embryo, Nonmammalian cytology
Epidermal Cells cytology
Female
Gene Expression Regulation, Developmental
Proto-Oncogene Proteins c-kit genetics
Signal Transduction
Stem Cell Factor genetics
Xenopus Proteins genetics
Xenopus laevis
Actin-Related Protein 2-3 Complex metabolism
Cilia physiology
Embryo, Nonmammalian physiology
Epidermal Cells physiology
Intercellular Junctions physiology
Proto-Oncogene Proteins c-kit metabolism
Stem Cell Factor metabolism
Xenopus Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1878-1551
- Volume :
- 56
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Developmental cell
- Publication Type :
- Academic Journal
- Accession number :
- 33756121
- Full Text :
- https://doi.org/10.1016/j.devcel.2021.02.026