Back to Search
Start Over
Molecular epidemiology of Mycobacterium tuberculosis complex in the Volta Region of Ghana.
- Source :
-
PloS one [PLoS One] 2021 Mar 17; Vol. 16 (3), pp. e0238898. Date of Electronic Publication: 2021 Mar 17 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- Context: Available molecular epidemiological data from recent studies suggest significant genetic variation between the different lineages of Mycobacterium tuberculosis complex (MTBC) and the MTBC lineages might have adapted to different human populations.<br />Aim: This study sought to determine the population structure of clinical MTBC isolates from the Volta Region of Ghana.<br />Methods: The MTBC isolates obtained from collected sputum samples were identified by PCR detecting of IS6110 and genotyped using spoligotyping. Non-tuberculous mycobacterial isolates were characterized by amplification of the heat shock protein 65 (hsp65) gene and sequencing. The drug susceptibility profiles of the MTBCs determined using GenoType MTBDRplus.<br />Results: One hundred and seventeen (117, 93.6%) out of 125 mycobacterial positive isolates were characterized as members of the MTBC of which M. tuberculosis sensu stricto (MTBss) and M. africanum (MAF) were respectively 94 (80.3%) and 23 (19.7%). In all, 39 distinct spoligotype patterns were obtained; 26 for MTBss and 13 for MAF lineages. Spoligotyping identified 89 (76%) Lineage 4, 16 (13.6%) Lineage 5, 7 (6.0%) Lineage 6, 3 (2.6%) Lineage 2, 1(0.9%) Lineage 3 and 1 (0.9%) Lineage 1. Among the Lineage 4 isolates, 62/89 (69.7%) belonged to Cameroon sub-lineage, 13 (14.7%) Ghana, 8 (9.0%) Haarlem, 2 (2.2%) LAM, 1 (1.1%) Uganda I, 1 (1.1%) X and the remaining two (2.2%) were orphan. Significant localization of MAF was found within the Ho municipality (n = 13, 29.5%) compared to the more cosmopolitan Ketu-South/Aflao (n = 3, 8.3%) (p-value = 0.017). Eight (8) non-tuberculous mycobacteria were characterized as M. abscessus (7) and M. fortuitum (1).<br />Conclusion: We confirmed the importance of M. africanum lineages as a cause of TB in the Volta region of Ghana.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Adult
Antitubercular Agents pharmacology
DNA, Bacterial genetics
DNA, Bacterial metabolism
Female
Genotype
Ghana epidemiology
Humans
Male
Microbial Sensitivity Tests
Middle Aged
Mycobacterium drug effects
Mycobacterium isolation & purification
Mycobacterium tuberculosis drug effects
Mycobacterium tuberculosis genetics
Mycobacterium tuberculosis isolation & purification
Nontuberculous Mycobacteria genetics
Nontuberculous Mycobacteria isolation & purification
Prevalence
Sputum microbiology
Tuberculosis microbiology
Tuberculosis pathology
Mycobacterium genetics
Tuberculosis epidemiology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 16
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 33730036
- Full Text :
- https://doi.org/10.1371/journal.pone.0238898