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Identification of the vibrational marker of tyrosine cation radical using ultrafast transient infrared spectroscopy of flavoprotein systems.
- Source :
-
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology [Photochem Photobiol Sci] 2021 Mar; Vol. 20 (3), pp. 369-378. Date of Electronic Publication: 2021 Feb 22. - Publication Year :
- 2021
-
Abstract
- Tryptophan and tyrosine radical intermediates play crucial roles in many biological charge transfer processes. Particularly in flavoprotein photochemistry, short-lived reaction intermediates can be studied by the complementary techniques of ultrafast visible and infrared spectroscopy. The spectral properties of tryptophan radical are well established, and the formation of neutral tyrosine radicals has been observed in many biological processes. However, only recently, the formation of a cation tyrosine radical was observed by transient visible spectroscopy in a few systems. Here, we assigned the infrared vibrational markers of the cationic and neutral tyrosine radical at 1483 and 1502 cm <superscript>-1</superscript> (in deuterated buffer), respectively, in a variant of the bacterial methyl transferase TrmFO, and in the native glucose oxidase. In addition, we studied a mutant of AppABLUF blue-light sensor domain from Rhodobacter sphaeroides in which only a direct formation of the neutral radical was observed. Our studies highlight the exquisite sensitivity of transient infrared spectroscopy to low concentrations of specific radicals.
- Subjects :
- Bacterial Proteins chemistry
Bacterial Proteins genetics
Bacterial Proteins metabolism
Cations chemistry
Flavoproteins metabolism
Glucose Oxidase chemistry
Glucose Oxidase metabolism
Methyltransferases chemistry
Methyltransferases genetics
Methyltransferases metabolism
Mutagenesis, Site-Directed
Photosynthetic Reaction Center Complex Proteins chemistry
Photosynthetic Reaction Center Complex Proteins genetics
Photosynthetic Reaction Center Complex Proteins metabolism
Recombinant Proteins biosynthesis
Recombinant Proteins chemistry
Recombinant Proteins isolation & purification
Rhodobacter sphaeroides metabolism
Flavoproteins chemistry
Free Radicals chemistry
Spectrophotometry, Infrared
Tyrosine chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1474-9092
- Volume :
- 20
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
- Publication Type :
- Academic Journal
- Accession number :
- 33721272
- Full Text :
- https://doi.org/10.1007/s43630-021-00024-y