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The effect of spike mutations on SARS-CoV-2 neutralization.
- Source :
-
Cell reports [Cell Rep] 2021 Mar 23; Vol. 34 (12), pp. 108890. Date of Electronic Publication: 2021 Mar 06. - Publication Year :
- 2021
-
Abstract
- Multiple severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines show protective efficacy, which is most likely mediated by neutralizing antibodies recognizing the viral entry protein, spike. Because new SARS-CoV-2 variants are emerging rapidly, as exemplified by the B.1.1.7, B.1.351, and P.1 lineages, it is critical to understand whether antibody responses induced by infection with the original SARS-CoV-2 virus or current vaccines remain effective. In this study, we evaluate neutralization of a series of mutated spike pseudotypes based on divergence from SARS-CoV and then compare neutralization of the B.1.1.7 spike pseudotype and individual mutations. Spike-specific monoclonal antibody neutralization is reduced dramatically; in contrast, polyclonal antibodies from individuals infected in early 2020 remain active against most mutated spike pseudotypes, but potency is reduced in a minority of samples. This work highlights that changes in SARS-CoV-2 spike can alter neutralization sensitivity and underlines the need for effective real-time monitoring of emerging mutations and their effect on vaccine efficacy.<br />Competing Interests: Declaration of interests Amsterdam UMC submitted a patent application on SARS-CoV-2 monoclonal antibodies, some of which were used in this study.<br /> (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Antibodies, Monoclonal genetics
Antibodies, Monoclonal immunology
Antibodies, Viral immunology
Antibody Formation
COVID-19 immunology
COVID-19 metabolism
COVID-19 Vaccines immunology
HEK293 Cells
Humans
Neutralization Tests methods
Point Mutation
Receptors, Virus genetics
Receptors, Virus metabolism
Spike Glycoprotein, Coronavirus immunology
Antibodies, Neutralizing immunology
COVID-19 virology
SARS-CoV-2 genetics
SARS-CoV-2 immunology
Spike Glycoprotein, Coronavirus genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2211-1247
- Volume :
- 34
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 33713594
- Full Text :
- https://doi.org/10.1016/j.celrep.2021.108890