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An Activatable Near-Infrared Fluorescence Hydrogen Sulfide (H 2 S) Donor for Imaging H 2 S Release and Inhibiting Inflammation in Cells.
- Source :
-
Analytical chemistry [Anal Chem] 2021 Mar 23; Vol. 93 (11), pp. 4894-4901. Date of Electronic Publication: 2021 Mar 10. - Publication Year :
- 2021
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Abstract
- Hydrogen sulfide (H <subscript>2</subscript> S) is a vital endogenous signal molecule that exerts critical physiological functions such as biological regulation and cytoprotection. Despite significant progress in developing H <subscript>2</subscript> S donors, site-specific delivery and controllable release of H <subscript>2</subscript> S in biological systems remain a key challenge. Herein, we develop new Cys-triggered fluorescent H <subscript>2</subscript> S donor Pro-S that is composed of a dicyanoisophorone-based near-infrared (NIR) fluorescent dye and a thiocarbamate moiety. The H <subscript>2</subscript> S donor releases H <subscript>2</subscript> S under the attack of Cys, accompanied by the release of a fluorescent reporter, which enables the real-time capturing of H <subscript>2</subscript> S by fluorescence spectroscopy or microscopy. Pro-S exhibits strong NIR fluorescence enhancement (70-fold), excellent controllable H <subscript>2</subscript> S release (30 min), high H <subscript>2</subscript> S release efficiency (62%), and well live-cell compatibility, allowing for visualization of H <subscript>2</subscript> S release in cells and zebrafish. Moreover, Pro-S presents a good effect of anti-inflammation in RAW 264.7 cells. This work provides a new idea for the design of H <subscript>2</subscript> S donors, which may be beneficial to the comprehension of the potential mechanism of inflammation and optimization of treatment strategies.
- Subjects :
- Animals
Fluorescent Dyes
HeLa Cells
Humans
Inflammation
Zebrafish
Hydrogen Sulfide
Subjects
Details
- Language :
- English
- ISSN :
- 1520-6882
- Volume :
- 93
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Analytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 33689305
- Full Text :
- https://doi.org/10.1021/acs.analchem.0c05081