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Evolution of organoid technology: Lessons learnt in Co-Culture systems from developmental biology.

Authors :
Zahmatkesh E
Khoshdel-Rad N
Mirzaei H
Shpichka A
Timashev P
Mahmoudi T
Vosough M
Source :
Developmental biology [Dev Biol] 2021 Jul; Vol. 475, pp. 37-53. Date of Electronic Publication: 2021 Mar 06.
Publication Year :
2021

Abstract

In recent years, the development of 3D organoids has opened new avenues of investigation into development, physiology, and regenerative medicine. Organoid formation and the process of organogenesis share common developmental pathways; thus, our knowledge of developmental biology can help model the complexity of different organs to refine organoids into a more sophisticated platform. The developmental process is strongly dependent on complex networks and communication of cell-cell and cell-matrix interactions among different cell populations and their microenvironment, during embryogenesis. These interactions affect cell behaviors such as proliferation, survival, migration, and differentiation. Co-culture systems within the organoid technology were recently developed and provided the highly physiologically relevant systems. Supportive cells including various types of endothelial and stromal cells provide the proper microenvironment, facilitate organoid assembly, and improve vascularization and maturation of organoids. This review discusses the role of the co-culture systems in organoid generation, with a focus on how knowledge of developmental biology has directed and continues to shape the development of more evolved 3D co-culture system-derived organoids.<br />Competing Interests: Declaration of competing interest The authors declare no conflicts of interest.<br /> (Copyright © 2021 Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1095-564X
Volume :
475
Database :
MEDLINE
Journal :
Developmental biology
Publication Type :
Academic Journal
Accession number :
33684433
Full Text :
https://doi.org/10.1016/j.ydbio.2021.03.001