Back to Search
Start Over
Chemopreventive effect of galangin against benzo(a)pyrene-induced stomach tumorigenesis through modulating aryl hydrocarbon receptor in Swiss albino mice.
- Source :
-
Human & experimental toxicology [Hum Exp Toxicol] 2021 Sep; Vol. 40 (9), pp. 1434-1444. Date of Electronic Publication: 2021 Mar 05. - Publication Year :
- 2021
-
Abstract
- The present study was aimed to evaluate the chemopreventive potential of galangin against benzo(a)pyrene (BaP)-induced stomach carcinogenesis in Swiss albino mice. Stomach cancer was induced in experimental mice using BaP oral administration. The mice were treated with galangin (10 mg/kg b.wt.) before and during BaP administration. Oral administration of galangin at a dose of 10 mg/kg b.wt. significantly (p < 0.05) prevented the tumor incidence, tumor volume in the experimental animals. Further, galangin pretreatment prevents BaP-induced lipid peroxidation and restores BaP-mediated loss of cellular antioxidants status. It has also been found that galangin prevents BaP-induced activation of phase I detoxification enzymes. Furthermore, galangin pretreatment prevented the BaP-induced overexpression of cytochrome P450s isoform genes (CYP1A1, CYP1B1), aryl hydrocarbon receptor system (AhR, ARNT), transcriptional activators (CBP/p300, NF-kB), tumor growth factors, proto-oncogenes, invasion markers (TGFB, SRC-1, MYC, iNOS, MMP2, MMP9) and Phase II metabolic isoenzyme genes (GST) in the stomach tissue homogenate when compared to the control groups. The western blot results confirm that galangin (10 mg/kg. b.wt.) treatment significantly prevented the BaP-mediated expression of ArR, ARNT, and CYP1A1 proteins in the mouse stomach tissue. Therefore, the present results confirm that galangin prevents BaP-induced stomach carcinogenesis probably through modulating ArR and ARNT expression in the experimental mice.
- Subjects :
- Animals
Disease Models, Animal
Humans
Mice
Receptors, Aryl Hydrocarbon drug effects
Basic Helix-Loop-Helix Transcription Factors drug effects
Benzo(a)pyrene toxicity
Carcinogenesis drug effects
Cell Transformation, Neoplastic drug effects
Flavonoids pharmacology
Flavonoids therapeutic use
Stomach Neoplasms chemically induced
Stomach Neoplasms drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1477-0903
- Volume :
- 40
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Human & experimental toxicology
- Publication Type :
- Academic Journal
- Accession number :
- 33663268
- Full Text :
- https://doi.org/10.1177/0960327121997979