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Adherent cell remodeling on micropatterns is modulated by Piezo1 channels.
- Source :
-
Scientific reports [Sci Rep] 2021 Mar 03; Vol. 11 (1), pp. 5088. Date of Electronic Publication: 2021 Mar 03. - Publication Year :
- 2021
-
Abstract
- Adherent cells utilize local environmental cues to make decisions on their growth and movement. We have previously shown that HEK293 cells grown on the fibronectin stripe patterns were elongated. Here we show that Piezo1 function is involved in cell spreading. Piezo1 expressing HEK cells plated on fibronectin stripes elongated, while a knockout of Piezo1 eliminated elongation. Inhibiting Piezo1 conductance using GsMTx4 or Gd <superscript>3+</superscript> blocked cell spreading, but the cells grew thin tail-like extensions along the patterns. Images of GFP-tagged Piezo1 showed plaques of Piezo1 moving to the extrusion edges, co-localized with focal adhesions. Surprisingly, in non-spreading cells Piezo1 was located primarily on the nuclear envelope. Inhibiting the Rho-ROCK pathway also reversibly inhibited cell extension indicating that myosin contractility is involved. The growth of thin extrusion tails did not occur in Piezo1 knockout cells suggesting that Piezo1 may have functions besides acting as a cation channel.
- Subjects :
- Cations metabolism
Cell Surface Extensions genetics
Cell Surface Extensions metabolism
Fibronectins metabolism
Gene Knockout Techniques
Green Fluorescent Proteins genetics
Green Fluorescent Proteins metabolism
HEK293 Cells
Humans
Ion Channels genetics
Myosins metabolism
Nuclear Envelope metabolism
Transfection
Cell Adhesion genetics
Cell Movement genetics
Cell Shape genetics
Ion Channels metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 33658557
- Full Text :
- https://doi.org/10.1038/s41598-021-84427-y