Mesenchymal Stromal Cells Derived from Bone Marrow and Adipose Tissue: Isolation, Culture, Characterization and Differentiation.

Authors :: Freitas GP
Souza ATP
Lopes HB
Trevisan RLB
Oliveira FS
Fernandes RR
Ferreira FU
Ros FA
Beloti MM
Rosa AL
Source :: Bio-protocol [Bio Protoc] 2020 Feb 20; Vol. 10 (4), pp. e3534. Date of Electronic Publication: 2020 Feb 20 (Print Publication: 2020).
Publication Year :: 2020
Abstract: Since their discovery, mesenchymal stromal cells (MSCs) have received a lot of attention, mainly due to their self-renewal potential and multilineage differentiation capacity. For these reasons, MSCs are a useful tool in cell biology and regenerative medicine. In this article, we describe protocols to isolate MSCs from bone marrow (BM-MSCs) and adipose tissues (AT-MSCs), and methods to culture, characterize, and differentiate MSCs into osteoblasts, adipocytes, and chondrocytes. After the harvesting of cells from bone marrow by flushing the femoral diaphysis and enzymatic digestion of abdominal and inguinal adipose tissues, MSCs are selected by their adherence to the plastic tissue culture dish. Within 7 days, MSCs reach 70% confluence and are ready to be used in subsequent experiments. The protocols described here are easy to perform, cost-efficient, require minimal time, and yield a cell population rich in MSCs.<br />Competing Interests: Competing interestsThe authors declare no conflict of interest.<br /> (Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.)

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