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Metabolomics and lipidomics in Caenorhabditis elegans using a single-sample preparation.
- Source :
-
Disease models & mechanisms [Dis Model Mech] 2021 Apr 01; Vol. 14 (4). Date of Electronic Publication: 2021 Apr 27. - Publication Year :
- 2021
-
Abstract
- Comprehensive metabolomic and lipidomic mass spectrometry methods are in increasing demand; for instance, in research related to nutrition and aging. The nematode Caenorhabditis elegans is a key model organism in these fields, owing to the large repository of available C. elegans mutants and their convenient natural lifespan. Here, we describe a robust and sensitive analytical method for the semi-quantitative analysis of >100 polar (metabolomics) and >1000 apolar (lipidomics) metabolites in C. elegans, using a single-sample preparation. Our method is capable of reliably detecting a wide variety of biologically relevant metabolic aberrations in, for example, glycolysis and the tricarboxylic acid cycle, pyrimidine metabolism and complex lipid biosynthesis. In conclusion, we provide a powerful analytical tool that maximizes metabolic data yield from a single sample. This article has an associated First Person interview with the joint first authors of the paper.<br />Competing Interests: Competing interests The authors declare no competing or financial interests.<br /> (© 2021. Published by The Company of Biologists Ltd.)
Details
- Language :
- English
- ISSN :
- 1754-8411
- Volume :
- 14
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Disease models & mechanisms
- Publication Type :
- Academic Journal
- Accession number :
- 33653825
- Full Text :
- https://doi.org/10.1242/dmm.047746