Back to Search
Start Over
Daidzein Induces Intrinsic Pathway of Apoptosis along with ER α/β Ratio Alteration and ROS Production.
- Source :
-
Asian Pacific journal of cancer prevention : APJCP [Asian Pac J Cancer Prev] 2021 Feb 01; Vol. 22 (2), pp. 603-610. Date of Electronic Publication: 2021 Feb 01. - Publication Year :
- 2021
-
Abstract
- Background: Low risk of breast cancer is observed among females consuming a moderate quantity of soy throughout their life. The present study was conducted to evaluate the anticancer potential of Daidzein, one of the major Isoflavones in soy using Human breast cancer cells MCF-7.<br />Methods: MCF-7 were subjected to various doses of Daidzein treatment to determine the IC50 value. Onset of apoptosis was ascertained by AnnexinV assay and caspase 3/7 activity post treatment. Expression of pro-apoptotic protein Bax and anti-apoptotic protein Bcl2 was also assessed to further confirm apoptotic mode of cell death. ROS production post treatment with Daidzein was assessed to ascertain the apoptosis via intrinsic pathway. Expression of ER α and ER β was evaluated by western blot analysis.<br />Results: Human breast cancer cells MCF-7 were found to be sensitive to Daidzein treatment, with an IC50 value of 50µM. Increased percentage of treated cells stained with Annexin V confirmed apoptosis mediated cell death. Activity of Caspase 3/7 activity was found to be 1.4-fold higher in Daidzein treated cells than control cells, confirming apoptosis. Daidzein caused over expression of Bax and down-regulated expression of Bcl2. There has been an outburst of ROS in Daidzein treated cells indicating that Daidzein induces apoptosis via intrinsic pathway. A decrease in the expression of ER α and increase in levels of ER β has been observed which are conducive indicator of apoptosis.<br />Conclusions: In conclusion, the present study suggests that Daidzein induces apoptosis in MCF-7 cells by mitochondrial pathway along with lowering the ratio of ER α/β and an outburst of Reactive Oxygen Species(ROS).
- Subjects :
- Adenocarcinoma pathology
Cell Culture Techniques
Estrogen Receptor alpha metabolism
Estrogen Receptor beta metabolism
Humans
Inhibitory Concentration 50
MCF-7 Cells drug effects
Proto-Oncogene Proteins c-bcl-2 metabolism
Reactive Oxygen Species metabolism
bcl-2-Associated X Protein metabolism
Apoptosis drug effects
Breast Neoplasms pathology
Estrogen Receptor alpha drug effects
Estrogen Receptor beta drug effects
Isoflavones pharmacology
Phytoestrogens pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 2476-762X
- Volume :
- 22
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Asian Pacific journal of cancer prevention : APJCP
- Publication Type :
- Academic Journal
- Accession number :
- 33639680
- Full Text :
- https://doi.org/10.31557/APJCP.2021.22.2.603