Twice-daily sessions result in a greater muscle strength and a similar muscle hypertrophy compared to once-daily session in resistance-trained men.

Background: The present study investigated the mid-term effects of training muscle groups once- versus twice-daily on morphofunctional adaptations in trained men.
Methods: Participants were randomly assigned to 1 of 2 experimental groups: 1 daily session per muscle group (1S, N.=11), where every muscle group was trained once a day or 2 daily sessions per muscle group (2S, N.=12), where every muscle group was trained twice. Testing was conducted before intervention and after 8 weeks for maximal strength (1RM) and muscular endurance (60%1RM) for bench press and parallel back squat exercises, and muscle thickness (MT) of the biceps brachii, triceps brachii, vastus lateralis, anterior quadriceps and pectoralis major.
Results: The major findings were as follows: 1) the increase in 1RM back squat was significantly greater in 2S (∆=16.1%) compared to 1S (∆=7.8%) (P<0.05); 2) both groups significantly increased bench press 1RM (1S: ∆=4.6%; 2S: ∆=6.8%), back squat 60% 1RM (1S: ∆=19.0%; 2S: ∆=24.3%), bench press 60% 1RM (1S: ∆=15.4%; 2S: ∆=24.0%) and all MT outcomes (P<0.05 for all), with no differences between experimental groups (1S and 2S).
Conclusions: This study provides evidence that a twice-daily resistance training augments lower-body muscular strength; however, the daily frequency does not seem to have any additive effect on upper-body muscular strength, muscular endurance, and muscle hypertrophy in trained men.