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Efficient Production of 1,3-Propanediol from Diverse Carbohydrates via a Non-natural Pathway Using 3-Hydroxypropionic Acid as an Intermediate.
- Source :
-
ACS synthetic biology [ACS Synth Biol] 2021 Mar 19; Vol. 10 (3), pp. 478-486. Date of Electronic Publication: 2021 Feb 24. - Publication Year :
- 2021
-
Abstract
- 1,3-Propanediol (1,3-PDO) is a promising platform chemical used to manufacture various polyesters, polyethers, and polyurethanes. Microbial production of 1,3-PDO using non-natural producers often requires adding expensive cofactors such as vitamin B <subscript>12</subscript> , which increases the whole production cost. In this study, we proposed and engineered a non-natural 1,3-PDO synthetic pathway derived from acetyl-CoA, enabling efficient accumulation of 1,3-PDO in Escherichia coli without adding expensive cofactors. This functional pathway was established by introducing the malonyl-CoA-dependent 3-hydroxypropionic acid (3-HP) module and screening the key enzymes to convert 3-HP to 1,3-PDO. The best engineered strain can produce 2.93 g/L 1,3-PDO with a yield of 0.35 mol/mol glucose in shake flask cultivation (and 7.98 g/L in fed-batch fermentation), which is significantly higher than previous reports based on homoserine- or malate-derived non-natural pathways. We also demonstrated for the first time the feasibility of producing 1,3-PDO from diverse carbohydrates including xylose, glycerol, and acetate based on the same pathway. Thus, this study provides an alternative route for 1,3-PDO production.
- Subjects :
- Aldehyde Dehydrogenase genetics
Aldehyde Dehydrogenase metabolism
Coenzyme A-Transferases genetics
Coenzyme A-Transferases metabolism
Glycerol metabolism
Lactic Acid chemistry
Lactic Acid metabolism
Plasmids genetics
Plasmids metabolism
Propylene Glycols chemistry
Vitamin B 12 chemistry
Xylose metabolism
Escherichia coli metabolism
Glucose metabolism
Lactic Acid analogs & derivatives
Metabolic Engineering
Propylene Glycols metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2161-5063
- Volume :
- 10
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- ACS synthetic biology
- Publication Type :
- Academic Journal
- Accession number :
- 33625207
- Full Text :
- https://doi.org/10.1021/acssynbio.0c00486