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Efficient Production of 1,3-Propanediol from Diverse Carbohydrates via a Non-natural Pathway Using 3-Hydroxypropionic Acid as an Intermediate.

Authors :
Li Z
Wu Z
Cen X
Liu Y
Zhang Y
Liu D
Chen Z
Source :
ACS synthetic biology [ACS Synth Biol] 2021 Mar 19; Vol. 10 (3), pp. 478-486. Date of Electronic Publication: 2021 Feb 24.
Publication Year :
2021

Abstract

1,3-Propanediol (1,3-PDO) is a promising platform chemical used to manufacture various polyesters, polyethers, and polyurethanes. Microbial production of 1,3-PDO using non-natural producers often requires adding expensive cofactors such as vitamin B <subscript>12</subscript> , which increases the whole production cost. In this study, we proposed and engineered a non-natural 1,3-PDO synthetic pathway derived from acetyl-CoA, enabling efficient accumulation of 1,3-PDO in Escherichia coli without adding expensive cofactors. This functional pathway was established by introducing the malonyl-CoA-dependent 3-hydroxypropionic acid (3-HP) module and screening the key enzymes to convert 3-HP to 1,3-PDO. The best engineered strain can produce 2.93 g/L 1,3-PDO with a yield of 0.35 mol/mol glucose in shake flask cultivation (and 7.98 g/L in fed-batch fermentation), which is significantly higher than previous reports based on homoserine- or malate-derived non-natural pathways. We also demonstrated for the first time the feasibility of producing 1,3-PDO from diverse carbohydrates including xylose, glycerol, and acetate based on the same pathway. Thus, this study provides an alternative route for 1,3-PDO production.

Details

Language :
English
ISSN :
2161-5063
Volume :
10
Issue :
3
Database :
MEDLINE
Journal :
ACS synthetic biology
Publication Type :
Academic Journal
Accession number :
33625207
Full Text :
https://doi.org/10.1021/acssynbio.0c00486